Structural Biology by Mass Spectrometry: Mapping Protein Interaction Surfaces of Membrane Receptor Complexes with ICAT.
Summary of "Structural Biology by Mass Spectrometry: Mapping Protein Interaction Surfaces of Membrane Receptor Complexes with ICAT."
No Summary Available
Affiliation
Journal Details
This article was published in the following journal.
Name: Journal of molecular biology
ISSN: 1089-8638
Pages: 481-2
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21515283
- DOI: http://dx.doi.org/10.1016/j.jmb.2011.04.030
Medical and Biotech [MESH] Definitions
Tandem Mass Spectrometry
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
Gas Chromatography-mass Spectrometry
A microanalytical technique combining mass spectrometry and gas chromatography for the qualitative as well as quantitative determinations of compounds.
Protein Interaction Mapping
Methods for determining interaction between proteins.
Mass Spectrometry
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
Peptide Mapping
Analysis of PEPTIDES that are generated from the digestion or fragmentation of a protein or mixture of PROTEINS, by ELECTROPHORESIS; CHROMATOGRAPHY; or MASS SPECTROMETRY. The resulting peptide fingerprints are analyzed for a variety of purposes including the identification of the proteins in a sample, GENETIC POLYMORPHISMS, patterns of gene expression, and patterns diagnostic for diseases.
PubMed Articles
The past 10years have witnessed a dramatic proliferation in the availability of protein interaction data. However, for interaction mapping based on affinity purification coupled with mass spectrometry...
Mass spectrometry-based methods to study protein architecture and dynamics.
Mass spectrometry is now an in indispensable tool in the armamentarium of molecular biophysics, where it is used for tasks ranging from protein sequencing and mapping of post-translational modificatio...
Structural Mass Spectrometry of Proteins Using Hydroxyl Radical Based Protein Footprinting.
Structural mass spectrometry is a rapidly growing field with many applications in basic research and pharmaceutical drug development. Using hydroxyl radical based footprinting approaches mass spectrom...
Ion mobility-mass spectrometry for structural proteomics.
Ion mobility coupled to mass spectrometry has been an important tool in the fields of chemical physics and analytical chemistry for decades, but its potential for interrogating the structure of protei...
Structural Mass Spectrometry in Protein Therapeutics Discovery.
The protein therapeutics market is one of the highest growing segments of the pharmaceutical industry with an estimated global market value of $77 billion by 2011 (Global Protein Therapeutics Market r...
Clinical Trials
Study of Tissue Samples From Patients With Lymphoma
RATIONALE: Studying tissue samples from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer....
The purpose of this study is to evaluate the basis for the reported good effects of antioxidant supplementation. A blood test using Mass Spectrometry will measure DNA damage which is expec...
Analysis of Brain Tumors Using Matrix Assisted Laser Desorption/Ionization Mass Spectrometry
We are collecting brain tissue specimens and blood samples from patients at Vanderbilt University Medical Center who are undergoing intracranial surgery to remove brain tissue, including b...
Erlotinib in Treating Patients With Breast Cancer That Can Be Removed by Surgery
RATIONALE: Erlotinib may stop the growth of tumor cells by blocking some of the enzymes needed for cell growth. Giving erlotinib before surgery may make the tumor smaller and reduce the am...
Protein and RNA Expression Patterns in Predicting Response to Treatment in Patients With Lung Cancer
RATIONALE: Studying samples of tumor tissue and blood in the laboratory from patients with cancer may help doctors learn more about changes that occur in RNA and identify protein expressio...
