High-throughput neuraminidase substrate specificity study of human and avian influenza A viruses.
Summary of "High-throughput neuraminidase substrate specificity study of human and avian influenza A viruses."
Despite the importance of neuraminidase (NA) activity in effective infection by influenza A viruses, limited information exists about the differences of substrate preferences of viral neuraminidases from different hosts or from different strains. Using a high-throughput screening format and a library of twenty α2-3- or α2-6-linked para-nitrophenol-tagged sialylgalactosides, substrate specificity of NAs on thirty-seven strains of human and avian influenza A viruses was studied using intact viral particles. Neuraminidases of all viruses tested cleaved both α2-3- and α2-6-linked sialosides but preferred α2-3-linked ones and the activity was dependent on the terminal sialic acid structure. In contrast to NAs of other subtypes of influenza A viruses which did not cleave 2-keto-3-deoxy-d-glycero-d-galacto-nonulosonic acid (Kdn) or 5-deoxy Kdn (5d-Kdn), NAs of all N7 subtype viruses tested had noticeable hydrolytic activities on α2-3-linked sialosides containing Kdn or 5d-Kdn. Additionally, group 1 NAs showed efficient activity in cleaving N-azidoacetylneuraminic acid from α2-3-linked sialoside.
Affiliation
Department of Chemistry, University of California, Davis, CA 95616, USA.
Journal Details
This article was published in the following journal.
Name: Virology
ISSN: 1096-0341
Pages: 12-9
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21501853
- DOI: http://dx.doi.org/10.1016/j.virol.2011.03.024
Medical and Biotech [MESH] Definitions
Antibodies, Catalytic
Antibodies that can catalyze a wide variety of chemical reactions. They are characterized by high substrate specificity and share many mechanistic features with enzymes.
Substrate Specificity
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Pneumovirus
A genus of the family PARAMYXOVIRIDAE (subfamily PNEUMOVIRINAE) where the human and bovine virions have neither hemagglutinin nor neuraminidase activity. RESPIRATORY SYNCYTIAL VIRUS, HUMAN is the type species.
High-throughput Screening Assays
Rapid methods of measuring the effects of an agent in a biological or chemical assay. The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays.
Lactate Dehydrogenases
Alcohol oxidoreductases with substrate specificity for LACTIC ACID.
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