Advertisement
Advertise here Publish your press releases here Sponsor BioPortfolio
Follow us on Twitter Sign up for daily news and research emails Contributors wanted

Investigations into the Phenolic Constituents of Dog's Mercury (Mercurialis perennis L.) by LC-MS/MS and GC-MS analyses.

15:21 EDT 24th July 2014 | BioPortfolio

Summary of "Investigations into the Phenolic Constituents of Dog's Mercury (Mercurialis perennis L.) by LC-MS/MS and GC-MS analyses."


INTRODUCTION:
Dog's mercury (Mercurialis perennis L.) is a traditional European medicinal plant considered as a rich source of bioactive natural products. Yet phytochemical data of the plant are scant.
OBJECTIVE:
This study aimed to identify the hydrophilic phenolic constituents from M. perennis by aqueous and hydroalcoholic extraction.
METHODOLOGY:
Extracts of herbal parts were investigated in-depth by HPLC(DAD)-MS/MS and GC/MS analyses. In addition, a novel compound was isolated and fully characterised by 1- and 2D-NMR experiments.
RESULTS:
Several conjugates of caffeic, p-coumaric and ferulic acids together with glucaric or 2-hydroxyglutaric acids (depsides) were detected in the aqueous extracts from aerial plant parts by use of LC-MS/MS techniques as well UV-spectral data. By implementation of preparative chromatography on polyamide pretreated with formic acid followed by vacuum liquid chromatography on reversed-phase C(18) -silica, one of the predominant depsides was isolated as a pure compound. The NMR spectra ((1) H and (13) C NMR) together with 2D-hetereonuclear multiple bond correlation NMR experiments (gHMBC and gHSQC) and chiral GC investigation, allowed identification of this compound as (-)-(E)-caffeoyl-2-(R)-oxoglutarate. This structure was additionally supported by GC/MS data after silylation and methylation reactions. The hydroalcoholic extract from aerial parts was separated by solvent partition between ethyl acetate and n-butanol. The latter fraction (n-butanol) yielded a mixture of mono- and oligo-glycosides of kaempferol and quercetin, all of them being assigned by LC-MS/MS.
CONCLUSIONS:
The present investigation constitutes the first comprehensive report on the hydrophilic constituents of the rarely studied plant Mercurialis and thus completes the phytochemical knowledge on M. perennis. Copyright © 2011 John Wiley & Sons, Ltd.

Affiliation

WALA Heilmittel GmbH, R&D, Dorfstrasse 1, Bad Boll/Eckwaelden, 73087, Germany. peter.lorenz@wala.de.

Journal Details

This article was published in the following journal.

Name: Phytochemical analysis : PCA
ISSN: 1099-1565
Pages:

Links

PubMed Articles [7031 Associated PubMed Articles listed on BioPortfolio]

Stabilizing conditions of metal mercury in mercury sulfurization using a planetary ball mill.

This study used a non-heating process to stabilize mercury by mercury sulfurization. Elemental mercury and sulfur were mixed using planetary ball milling under various experimental conditions and the...

'Fishy' Make-up on the Hook for Mercury Exposure: A Case Series.

This report examines the source and outcome of four Barbadian women with extremely high hair mercury concentrations (361-5617 µg/g inorganic mercury) due to topical application of mercury containing...

Preliminary antimycobacterial study on selected Turkish plants (Lamiaceae) against Mycobacterium tuberculosis and search for some phenolic constituents.

The global resurgence of tuberculosis is a significant threat. Lamiaceae members have been used in folk remedies for centuries. This study was designed to assess the in-vitro antimycobacterial activit...

Allergological and toxicological aspects in a multiple chemical sensitivity cohort.

Background. Multiple chemical sensitivity (MCS) is a chronic condition characterized by an exaggerated response to toxicants. We ascertained the prevalence of allergy to metals and toxicological aspec...

Establishing a case for improved food phenolic analysis.

Future phenolic research findings using a multidisciplinary approach will ensure profitability and sustainability of specialty crop industries, while also improving the nutritional and economic choice...

Clinical Trials [410 Associated Clinical Trials listed on BioPortfolio]

Health Effects of Silver-Mercury Dental Fillings

The purpose of this study is to determine whether there are detectable health effects of low-level mercury exposure (from normal exposure to mercury-containing dental fillings) in the know...

Healthful Seafood Consumption for Sensitive Populations

Fish can provide pregnant women with omega-3 fatty acids for fetal brain development but some fish contains high levels of mercury which is detrimental to fetal brain development. The hypo...

Mercury Levels in Premature and LBW Infants Receiving Thimerosal-containing Vaccines

The goal of the study is to find out how much mercury is in the blood, urine, and stools of premature and low birth weight infants who have received standard childhood vaccines that are gi...

Measuring Blood Pressure in Pregnancy Using Mercury and Automated Device

The aim of the PRAM study was to determine whether women diagnosed with hypertension in pregnancy, using the traditional mercury device, have the same pregnancy outcomes when blood pressur...

Data Analyses for Ancillary WISE Femhrt Hormone Replacement Study

For the purposes of this study, as a core lab coordinating center, the investigators will be performing P31 MRS core lab analyses; hormone core lab analyses; lipid core lab analyses; gluco...

Medical and Biotech [MESH] Definitions

Stable mercury atoms that have the same atomic number as the element mercury, but differ in atomic weight. Hg-196, 198-201, and 204 are stable mercury isotopes.

Unstable isotopes of mercury that decay or disintegrate emitting radiation. Hg atoms with atomic weights 185-195, 197, 203, 205, and 206 are radioactive mercury isotopes.

A silver metallic element that exists as a liquid at room temperature. It has the atomic symbol Hg (from hydrargyrum, liquid silver), atomic number 80, and atomic weight 200.59. Mercury is used in many industrial applications and its salts have been employed therapeutically as purgatives, antisyphilitics, disinfectants, and astringents. It can be absorbed through the skin and mucous membranes which leads to MERCURY POISONING. Because of its toxicity, the clinical use of mercury and mercurials is diminishing.

Inorganic compounds that contain mercury as an integral part of the molecule.

Organic mercury compounds in which the mercury is attached to an ethyl group.

Search BioPortfolio: