Ultra-sensitive Quantification of Sub-nanomolar Levels of Iodine in Blood Serum Samples by Kinetic-spectrophotometric Method.
Summary of "Ultra-sensitive Quantification of Sub-nanomolar Levels of Iodine in Blood Serum Samples by Kinetic-spectrophotometric Method."
A simple and sensitive kinetic-spectrophotometric method is developed for the determination of trace amounts of iodine in blood serum samples based on its catalytic effect on the oxidation of Nile Blue A by potassium bromate in sulfuric acid medium and at 25°C. The absorbance is measured at 595.5 nm with the fixed-time method. The optimization of the operating conditions regarding concentration of the reagents, temperature, and interferences are also investigated. The calibration curve is linear over the concentration range between 20.0 to 500.0 ng ml(-1) of iodine with good precision and accuracy. The detection limit of the method is down to 12.0 ng ml(-1). The relative standard deviation for a standard solution of 100.0 ng ml(-1) of iodine is 1.32% (n = 10). The proposed method provides a highly sensitive, selective, and relatively rapid assay for iodine at ultra trace level without any pre-concentration and separation step. The method was applied to the determination of iodine in blood serum samples. The analytical results of the real samples were in excellent agreement with standard method.
Young Researchers Club, Hamedan Branch, Islamic Azad University, Hamedan, Iran, email@example.com.
This article was published in the following journal.
Name: Biological trace element research
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21732074
- DOI: http://dx.doi.org/10.1007/s12011-011-9126-6
Medical and Biotech [MESH] Definitions
Serum Albumin, Radio-iodinated
Normal human serum albumin mildly iodinated with radioactive iodine (131-I) which has a half-life of 8 days, and emits beta and gamma rays. It is used as a diagnostic aid in blood volume determination. (from Merck Index, 11th ed)
Hyperthyroxinemia, Familial Dysalbuminemic
An inherited autosomal dominant trait characterized by abnormally elevated levels of total serum THYROXINE; (T4) in euthyroid patients with abnormal SERUM ALBUMIN that binds T4 with enhanced affinity. The serum levels of free T4, free T3, and TSH are normal. It is one of several T4 abnormalities produced by non-thyroid disorder. This condition is due to mutations of the ALB gene on CHROMOSOME 4.
Stable iodine atoms that have the same atomic number as the element iodine, but differ in atomic weight. I-127 is the only naturally occurring stable iodine isotope.
Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
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