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Preparative separation of lithospermic acid B from Salvia miltiorrhiza by polyamide resin and preparative high-performance liquid chromatography.

12:19 EDT 24th April 2014 | BioPortfolio

Summary of "Preparative separation of lithospermic acid B from Salvia miltiorrhiza by polyamide resin and preparative high-performance liquid chromatography."

Adsorption on polyamide resin was investigated as a means of separating lithospermic acid B (LAB) from a crude extract of the roots of the traditional Chinese medicine Salvia miltiorrhiza Bunge ("Danshen"). Variables affecting adsorption capacity (solution pH, contact time on resin, initial LAB concentration) were studied. Adsorption was strongly dependent upon the initial concentration of LAB and pH. In all conditions, the polyamide resin gave optimal adsorption of LAB at an initial concentration of 2.66mg/mL and pH <3.0. The adsorption isotherm correlated well with the Langmuir-type adsorption isotherm. Maximal adsorption capacity was calculated to be 380mg/g at pH 2.0 and 25°C. LAB purity of 85.30% could be obtained by polyamide resin adsorption followed by elution with 70% ethanol solution, and the recovery was 87.1%. After preparative HPLC, the maximum HPLC purity obtained was 99.28% with a recovery of 75.2%. This method provides an efficient and low-cost method for LAB purification for industrial applications.

Affiliation

School Pharmaceutical Engineering, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 116016, China.

Journal Details

This article was published in the following journal.

Name: Journal of chromatography. A
ISSN: 1873-3778
Pages: 4606-11

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Medical and Biotech [MESH] Definitions

A plant species which is known as an Oriental traditional medicinal plant.

A plant species of the Salvia genus known as a spice and medicinal plant.

A plant genus of the family BORAGINACEAE. Members contain lithospermans and lithospermic acid.

Cyclic amide of caproic acid used in manufacture of synthetic fibers of the polyamide type. Can cause local irritation.

The separation of individuals or groups resulting in the lack of or minimizing of social contact and/or communication. This separation may be accomplished by physical separation, by social barriers and by psychological mechanisms. In the latter, there may be interaction but no real communication.

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