Advertisement

Topics

Determination of minimum inhibitory and minimum bactericidal concentrations of tiamulin against field isolates of Actinobacillus pleuropneumoniae.

Summary of "Determination of minimum inhibitory and minimum bactericidal concentrations of tiamulin against field isolates of Actinobacillus pleuropneumoniae."

Tiamulin activity was measured against 19 UK field isolates of Actinobacillus pleuropneumoniae collected between 2003 and 2009 and the type strain ATCC 27090 as a control, with the intention of comparing broth with serum as growth media. Broth microdilution MIC/MBC tests were performed in accordance with the Clinical and Laboratory Standards Institute (CLSI) guideline M31-A3, in 'Veterinary Fastidious Medium' (VFM) (supplemented Mueller-Hinton broth at pH 7.3) and in 100% swine serum. For improved precision, a modified, overlapping doubling-dilution series was used (tiamulin concentration range 0.3-72μg/ml). The MBC was reported as the lowest concentration producing a 99.9% reduction in bacterial density in the sub-cultured well contents, relative to the starting inoculum. The mean MBC/MIC ratio for tiamulin against A. pleuropneumoniae in VFM was low (1.74:1), even though tiamulin is classed as a bacteriostatic drug. Only three of the 19 isolates and the reference strain grew in 100% serum and their MICs were higher than those determined in VFM. It is postulated that this difference was due to differences in pH of the matrices or binding of tiamulin to serum proteins or a combination of both factors.

Affiliation

Don Whitley Scientific Ltd., Shipley, West Yorkshire, BD177SE, UK.

Journal Details

This article was published in the following journal.

Name: Veterinary microbiology
ISSN: 1873-2542
Pages: 409-12

Links

DeepDyve research library

PubMed Articles [10604 Associated PubMed Articles listed on BioPortfolio]

Short communication: Determination of the ability of Thymox to kill or inhibit various species of microorganisms associated with infectious causes of bovine lameness in vitro.

Infectious claw diseases continue to plague cattle in intensively managed husbandry systems. Poor foot hygiene and constant moist environments lead to the infection and spread of diseases such as digi...

Antibacterial Activity of Carum copticum Essential Oil Against Escherichia Coli O157:H7 in Meat: Stx Genes Expression.

This work were aimed to (a) determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Carum copticum essential oil (EO) against Escherichia. coli O157:H7 ...

Observations of resistance through minimum inhibitory concentrations trends for respiratory specimens of commonly isolated organisms.

The objective of this study was to determine minimum inhibitory concentration (MIC) trends among common bacterial organisms found in respiratory isolates in the trauma intensive care unit setting.

Antimicrobial Activities of Methanol, Ethanol and Supercritical CO2 Extracts of Philippine Piper betle L. on Clinical Isolates of Gram Positive and Gram Negative Bacteria with Transferable Multiple Drug Resistance.

Piper betle L. has traditionally been used in alternative medicine in different countries for various therapeutic purposes, including as an anti-infective agent. However, studies reported in the liter...

Antibacterial Effects of Pyrolysis Oil Against Salmonella Typhimurium and Escherichia coli.

Many issues have been found to be related to food preservation and food contamination caused by various pathogenic bacteria in recent years. Many antibacterial agents act efficiently against Gram-posi...

Clinical Trials [1735 Associated Clinical Trials listed on BioPortfolio]

Development of an Adjustment Assistance Tool Dosage of Fluoroquinolones in a Population Pharmacokinetic Model

Fluoroquinolones (FQ) are among pivotal antibiotic treatments in difficult-to-treat infections. Their efficacy has been shown to be linked to the ratio area under the curve (AUC) of their ...

Zostavax® at Minimum Release Specification Approaching Expiry

Primary objective: To demonstrate whether or not ZOSTAVAX® at minimum release specification approaching expiry potency elicits an acceptable Varicella-Zoster Virus (VZV) antibody fold ri...

Treatment of Arthritis With Syk Kinase Inhibition (TASKI-1)

This is a Phase II, multicenter, randomized, double-blind, placebo-controlled, ascending dose, dose ranging study to evaluate up to three doses of R935788 (50 mg bid, 100 mg bid and 150 m...

Weight Change With Controlled Ankle Movement (CAM) Walker Boot Use

The purpose of the study is to determine if patients wearing a CAM walker boot for a minimum of 6 weeks have a change in weight compared to patients who wear a CAM boot for a minimum of 6 ...

Comparison of CXR and MnDCT

A comparison of chest x-ray (CXR) and Minimum dose CT (MnDCT) in the acutely ill patient. The hypothesis is that MnDCT is more sensitive than CXR in the detection of acute findings in the ...

Medical and Biotech [MESH] Definitions

The minimum amount of stimulus energy necessary to elicit a sensory response.

The minimum concentration at which taste sensitivity to a particular substance or food can be perceived.

The capacity of an organization, institution, or business to produce desired results with a minimum expenditure of energy, time, money, personnel, materiel, etc.

The minimum acceptable patient care, based on statutes, court decisions, policies, or professional guidelines.

Irregular HEART RATE caused by abnormal function of the SINOATRIAL NODE. It is characterized by a greater than 10% change between the maximum and the minimum sinus cycle length or 120 milliseconds.

Quick Search
Advertisement
 


DeepDyve research library

Relevant Topic

Pharmacy
Pharmacy is the science and technique of preparing as well as dispensing drugs and medicines. It is a health profession that links health sciences with chemical sciences and aims to ensure the safe and effective use of pharmaceutical drugs. The scope of...


Searches Linking to this Article