Track topics on Twitter Track topics that are important to you
Aims The present study aims to investigate the interaction between nitric oxide (NO) and hydrogen sulfide (H(2)S), the two important gaseous mediators in rat hearts. Methods and Results Intracellular calcium in isolated cardiomyocytes was measured with a spectrofluorometric method using fura-2. Myocyte contractility was measured with a video edge system. NaHS (50 muM, an H(2)S donor) had no significant effect on the resting calcium level, electrically-induced calcium transients and cell contractility in ventricular myocytes. Stimulating endogenous NO production with L-arginine or exogenous application of NO donors [sodium nitroprusside (SNP) and 2-(N,N-Diethylamino)-diazenolate-2-oxide (DEA/NO)] decreased myocyte twitch amplitudes accompanied by slower velocities of both cell contraction and relaxation. Surprisingly, NaHS reversed the negative inotropic and lucitropic effects of the above three NO increasing agents. In addition, the mixture of SNP+NaHS increased, whereas SNP alone decreased, the resting calcium level and the amplitudes of electrically-induced calcium transients. Angeli's salt, a nitroxyl anion (HNO) donor, mimicked the effect of SNP+NaHS on calcium handling and myocyte contractility. Three thiols, N-acetyl-cysteine, L-cysteine and glutathione, abolished the effects of HNO and SNP+NaHS on myocyte contraction. Neither Rp-cAMP [100 muM, a protein kinase A (PKA) inhibitor] nor Rp-cGMP ([10 muM, a protein kinase G (PKG) inhibitor]) affected the effects of SNP+NaHS, suggesting a cAMP/PKA or cGMP/PKG independent mechanism. Conclusions H(2)S may interact with NO to form HNO which produces positive inotropic and lusitropic effects. Our findings may shed a light on the interaction of NO and H(2)S and provide new clues to treat cardiovascular diseases.
Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, 117597, Singapore.
This article was published in the following journal.
Name: Cardiovascular research
Hydrogen sulfide (H2S) is the third gasotransmitter recently discovered after nitric oxide (NO) and carbon monoxide. Both NO and H2S are involved in multiple physiological functions. Whereas NO has be...
There are several reviews on nitric oxide (NO) and hydrogen sulfide (H2 S) and their role in vascular diseases in the current relevant literature. The aim of this review has been to discuss, within th...
Nitric oxide (NO) is a bioactive gas that has multiple roles in innate and adaptive immune responses. In macrophages, nitric oxide is produced by inducible nitric oxide synthase upon microbial and cyt...
As the third confirmed gaseous transmitters, hydrogen sulfide was found to play a vital role in the eternal milieu both physiologically and pathologically. What's intriguing is that, there exists a de...
Nitric oxide (NO) is a major endogenous regulator of vascular tone. Inhaled nitric oxide (iNO) gas has been investigated as treatment for persistent pulmonary hypertension of the newborn.
This blinded, placebo-controlled study will administer inhaled nitric oxide to patients undergoing liver transplantation. The purpose of the study is to test if inhaled nitric oxide preven...
A minimum of 100 patients will be enrolled in the study to demonstrate which diagnostic treatment (oxygen or nitric oxide) is most capable of identifying patients with a reactive pulmonary...
The purpose of this study is to determine whether inhaled nitric oxide is an effective treatment for microcirculatory dysfunction and acute organ system failure in the early stage of sepsi...
This study is a non-randomized, Phase 2 clinical trial designed to measure the cerebral blood flow (CBF) response to inhaled nitric oxide in acute ischemic stroke patients and healthy subj...
The purpose of this study is to look at the long term consequences of prematurity in infants treated with inhaled nitric oxide (iNO) while in the neonatal intensive care unit.
A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.
A diverse group of agents, with unique chemical structures and biochemical requirements, which generate NITRIC OXIDE. These compounds have been used in the treatment of cardiovascular diseases and the management of acute myocardial infarction, acute and chronic congestive heart failure, and surgical control of blood pressure. (Adv Pharmacol 1995;34:361-81)
An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.
A CALCIUM-dependent, constitutively-expressed form of nitric oxide synthase found primarily in ENDOTHELIAL CELLS.
A CALCIUM-dependent, constitutively-expressed form of nitric oxide synthase found primarily in NERVE TISSUE.
Cardiovascular disease (CVD)
Acute Coronary Syndromes (ACS) Blood Cardiovascular Dialysis Hypertension Stent Stroke Vascular Cardiovascular disease (CVD) includes all the diseases of the heart and circulation including coronary heart disease (angina...
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...