Investigation of the absorption mechanism of solubilized curcumin using caco-2 cell monolayers.

Summary of "Investigation of the absorption mechanism of solubilized curcumin using caco-2 cell monolayers."

Curcumin is a bioactive compound with poor oral bioavailability. Low water solubility and rapid metabolism are two known limiting factors, but the absorption mechanism of solubilized curcumin remains unclear. This study investigated the permeation mechanism of solubilized curcumin using an in vitro Caco-2 cell monolayer model. It was shown that curcumin permeated across the monolayers fairly rapidly [P(app)(A-B) = (7.1 ± 0.7) × 10(-6) cm/s] and the permeation mechanism was found as passive diffusion [P(app)(B-A)/P(app)(A-B) = 1.4]. Furthermore, the permeation rates of curcumin complexed with bovine serum albumin and in the bile salts-fatty acids mixed micelles were also determined as P(app)(mixed micelle) > P(app)(DMSO) > P(app)(protein complex). These results suggested that solubilization agents play an important role in the permeation of solubilized curcumin, and stronger binding between the solubilization agents and curcumin may decrease the permeation rate. The results further suggest that lipid-based formulations, which solubilize curcumin in mixed micelles after lipid digestion, are promising vehicles for curcumin oral delivery.


Department of Food Science, Rutgers, the State University of New Jersey , 65 Dudley Road, New Brunswick, New Jersey 08901, United States.

Journal Details

This article was published in the following journal.

Name: Journal of agricultural and food chemistry
ISSN: 1520-5118
Pages: 9120-6


DeepDyve research library

PubMed Articles [28651 Associated PubMed Articles listed on BioPortfolio]

A comprehensive approach to ascertain the binding mode of curcumin with DNA.

Curcumin is a natural phytochemical from the rhizoma of Curcuma longa, the popular Indian spice that exhibits a wide range of pharmacological properties like antioxidant, anticancer, anti-inflammatory...

Elucidation of the Intestinal Absorption Mechanism of Celastrol Using the Caco-2 Cell Transwell Model.

Brush border membrane vesicle and Caco-2 cell line: Two experimental models for evaluation of absorption enhancing effects of saponins, bile salts, and some synthetic surfactants.

The aim of this study was to investigate the influence of absorption enhancers in the uptake of hydrophilic compounds. The permeation of the two hydrophilic drug models gentamicin and 5 (6)-carboxyflu...

Positively Charged Surface-Modified Solid Lipid Nanoparticles Promote the Intestinal Transport of Docetaxel through Multifunctional Mechanisms in Rats.

Solid lipid nanoparticles (SLNs) are one of the most promising nanocarriers to increase the oral absorption of drugs with poor solubility and low permeability. However, the absorption mechanism of SLN...

Establishment and characterization of a novel Caco-2 subclone with a similar low expression level of human carboxylesterase 1 to human small intestine.

Caco-2 cells predominantly express human carboxylesterase 1 (hCE1), unlike the human intestine which predominantly expresses human carboxylesterase 2 (hCE2). Transport experiments using Caco-2 cell mo...

Clinical Trials [7408 Associated Clinical Trials listed on BioPortfolio]

Bio-availability of a New Liquid Tumeric Extract

Curcumin is a commonly-used spice and food coloring. Evidence suggests that curcumin can suppress tumor initiation, promotion and metastasis in a variety of tumor cell lines. The current a...

A Study to Compare the Pharmacokinetic Profile of a Proprietary Curcumin Forumulation to a Comparator Curcumin Product

This study is investigating the pharmacokinetic profile (i.e. the way the product is processed by the body) of a proprietary curcumin formulation compared to an unformulated curcumin produ...

Curcumin (Diferuloylmethane Derivative) With or Without Bioperine in Patients With Multiple Myeloma

Primary Objectives: 1. To evaluate clinical tolerance and response to curcumin alone and in combination with Bioperine in patients with multiple myeloma. 2. To compare the...

Pharmacokinetics of Curcumin in Healthy Volunteers

This study looks to describe the pharmacokinetics of curcumin delivered as a single oral dose in healthy female volunteers. The impact of piperine and silybin on the pharmacokinetics of cu...

Trial of Curcumin in Advanced Pancreatic Cancer

The goal of this clinical research study is to learn if treatment with curcumin can help shrink or slow the growth of pancreatic cancers. The effect of curcumin on the way pancreatic canc...

Medical and Biotech [MESH] Definitions

A yellow-orange dye obtained from tumeric, the powdered root of CURCUMA longa. It is used in the preparation of curcuma paper and the detection of boron. Curcumin appears to possess a spectrum of pharmacological properties, due primarily to its inhibitory effects on metabolic enzymes.

Dynamic and kinetic mechanisms of exogenous chemical and drug ABSORPTION; BIOLOGICAL TRANSPORT; TISSUE DISTRIBUTION; BIOTRANSFORMATION; elimination; and TOXICOLOGY as a function of dosage, and rate of METABOLISM. It includes toxicokinetics, the pharmacokinetic mechanism of the toxic effects of a substance. ADME and ADMET are short-hand abbreviations for absorption, distribution, metabolism, elimination and toxicology.

Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation.

Surface ligands that mediate cell-to-cell adhesion and function in the assembly and interconnection of the vertebrate nervous system. These molecules promote cell adhesion via a homophilic mechanism. These are not to be confused with NEURAL CELL ADHESION MOLECULES, now known to be expressed in a variety of tissues and cell types in addition to nervous tissue.

Agents that aid or increase the action of the principle drug (DRUG SYNERGISM) or that affect the absorption, mechanism of action, metabolism, or excretion of the primary drug (PHARMACOKINETICS) in such a way as to enhance its effects.

Quick Search

DeepDyve research library

Relevant Topic

Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Searches Linking to this Article