Cellular Compatibility of Biomineralized ZnO Nanoparticles Based on Prokaryotic and Eukaryotic Systems.
Summary of "Cellular Compatibility of Biomineralized ZnO Nanoparticles Based on Prokaryotic and Eukaryotic Systems."
Zinc oxide nanoparticles (NPs) with the size of ~100 nm were prepared via a facile biomineralization process in the template of silk fibroin (SF) peptide at room temperature. These ZnO NPs have shown a remarkable behavior that they were low toxic to Gram-positive bacteria (Staphylococcus aureus, Staphylococcus agalactiae), Gram-negative bacteria (Escherichia coli) and eukaryotic cells (mouse L929 fibroblasts). Bacteriological test indicated that ZnO NPs presented 50% inhibitory effect on Streptococcus agalactiae at the concentrations of >100 mM, whereas at the same concentrations the growth of Staphylococcus aureus and Escherichia coli were hardly inhibited. On the other hand, a remarkable proliferation of Staphylococcus aureus or Escherichia coli was observed at the concentrations of ZnO NPs <50 mM. Moreover, the cytotoxicity test demonstrated that ZnO NPs mineralized with SF peptide possessed a low toxicity to mouse L929 fibroblasts. The SF peptide coated on the surface of ZnO NPs permitted greater adhesion and greater proliferation of mouse L929 fibroblasts consequently. Besides, from TEM micrographs of the cell ultrastructure, endocytosis of NPs into the cytoplasm can be detected and the ultrastructure of the cell underwent few changes. The cell membrane retained integrity, euchromatin dispersed homogenously inside the cytoplasm, the mitochondrial architecture kept intact and none of intracellular vacuole was observed. High-resolution transmission electron microscopy images and selected area electron diffraction patterns of the cell ultrathin sections indicated that the crystal structure of NPs was not damaged by the organelle or cytoplasm. All these observations indicated that ZnO NPs mineralized with the SF peptide possess good cytocompatibility.
This article was published in the following journal.
Name: Langmuir : the ACS journal of surfaces and colloids
Medical and Biotech [MESH] Definitions
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
Cold Shock Proteins And Peptides
Cellular proteins and peptides that are induced in response to cold stress. They are found in a broad variety of prokaryotic and eukaryotic organisms.
Prokaryotic Initiation Factors
Peptide initiation factors from prokaryotic organisms. Only three factors are needed for translation initiation in prokaryotic organisms, which occurs by a far simpler process than in PEPTIDE CHAIN INITIATION, TRANSLATIONAL of eukaryotic organisms.
Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Escherichia coli is the most widely used host for producing membrane proteins. Thus far, to study the consequences of membrane protein overexpression in E. coli, we have focussed on prokaryotic membra...
Mounting evidence in recent years has challenged the dogma that prokaryotes are simple and undefined cells devoid of an organized subcellular architecture. In fact, proteins once thought to be the pur...
The prokaryotic and eukaryotic homologues of complex I (proton-pumping NADH:quinone oxidoreductase) perform the same function in energy transduction, but the eukaryotic enzymes are twice as big as the...
Promoters are modular DNA structures containing complex regulatory elements required for gene transcription initiation. Hence, the identification of promoters using machine learning approach is very i...
Recent studies point to a diverse assemblage of prokaryotic cognates of the eukaryotic ubiquitin (Ub) system. These systems span an entire spectrum, ranging from those catalyzing cofactor and amino ac...
The aim of this study is to evaluate the antibacterial activity of crosslinked quaternary ammonium polyethylenimine (PEI) nanoparticles incorporated at 1-2% w/w in a commercial soft liner...
The effect of Antibacterial Nanoparticles, Incorporated in cement, on S.mutans in the margins of provisional restorations is going to be examined clinically by using two kinds of provision...
Bloodstream infections are common in Intensive Care Units (ICUs). The need of a central venous line increases the risk of bacteremia and central venous catheter (CVC) related infections. T...
The overall goal of this study was to implement and evaluate the effects of a systems-based and pharmacist-mediated program designed to improve adherence for patients with known coronary h...
This study examines the efficacy of two computer-based training systems to teach children with ADHD to attend more effectively.