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Complete genome sequence of a dahlia common mosaic virus isolate from New Zealand.

14:48 EDT 21st May 2013 | BioPortfolio

Summary of "Complete genome sequence of a dahlia common mosaic virus isolate from New Zealand."

Dahlia mosaic disease of the ornamental flowering plant Dahlia is caused by two caulimoviruses, dahlia mosaic virus (DMV) and dahlia common mosaic virus (DCMV). We used a rolling-circle amplification method to amplify, clone and determine for the first time the full genome sequence of a DCMV isolate from New Zealand (DCMV-NZ). Within the 7949-bp circular double-stranded retro-transcribing DCMV-NZ DNA, we identified six putative open reading frames, typical of all genomes in the family Caulimoviridae. The availability of the complete DCMV sequence provides a reference genome against which all others can be compared.

Affiliation

School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand.

Journal Details

This article was published in the following journal.

Name: Archives of virology
ISSN: 1432-8798
Pages:

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Medical and Biotech [MESH] Definitions

Comovirus

A genus of plant viruses of the family COMOVIRIDAE in which the bipartite genome is encapsidated in separate icosahedral particles. Mosaic and mottle symptoms are characteristic, and transmission is exclusively by leaf-feeding beetles. Cowpea mosaic virus is the type species.

Tobacco Mosaic Satellite Virus

A spherical RNA satellite virus which requires an obligatory helper TOBACCO MOSAIC VIRUS for replication.

Human Genome Project

A coordinated effort of researchers to map (CHROMOSOME MAPPING) and sequence (SEQUENCE ANALYSIS, DNA) the human GENOME.

Cucumovirus

A genus of plant viruses of the family BROMOVIRIDAE, which infect cucurbits and solanaceous plants. Transmission occurs via aphids in a non-persistent manner, and also via seeds. The type species Cucumber mosaic virus, a CUCUMOVIRUS, should not be confused with Cucumber green mottle mosaic virus, a TOBAMOVIRUS.

Sequence Tagged Sites

Short tracts of DNA sequence that are used as landmarks in GENOME mapping. In most instances, 200 to 500 base pairs of sequence define a Sequence Tagged Site (STS) that is operationally unique in the human genome (i.e., can be specifically detected by the polymerase chain reaction in the presence of all other genomic sequences). The overwhelming advantage of STSs over mapping landmarks defined in other ways is that the means of testing for the presence of a particular STS can be completely described as information in a database.

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