Determination of benzoyl peroxide, as benzoic acid, in wheat flour by capillary electrophoresis compared with HPLC.
Summary of "Determination of benzoyl peroxide, as benzoic acid, in wheat flour by capillary electrophoresis compared with HPLC."
BACKGROUND:
In recent years, debate on the addition of benzoyl peroxide (BP) to wheat flour has increased in China. Medical studies have so far not confirmed that BP in wheat flour causes definite damage to the human body, but its main metabolite in the human body is benzoic acid. The addition of BP to wheat flour has been forbidden in China since 1 May 2011. It is therefore necessary to develop a suitable method to determine BP in wheat flour.
RESULTS:
A simple method using capillary electrophoresis (CE) was developed for determination of BP in wheat flour. BP was determined as benzoic acid after preceding reduction by potassium iodide. Separation was completed in less than 7 min with a running buffer of 10 mmol L(-1) sodium borate (pH 9.18), 4 s hydrodynamic injection, 25 kV separation voltage and UV detection at 228 nm. The method was validated in terms of linearity, sensitivity, detection limit, precision and accuracy. The detection limit was 0.29 µg mL(-1) . The method was successfully applied to the determination of BP in wheat flour samples, with recoveries between 96.1% and 102.6%.
CONCLUSION:
The performance of the CE method was comparable, and the quantitative results were in good agreement with those using high-performance liquid chromatography (HPLC). Meanwhile the proposed CE method has the advantages of better resolution, shorter analysis time and lower cost, and would be a good alternative to HPLC for routine monitoring of BP amount in wheat flour. Copyright © 2011 Society of Chemical Industry.
Affiliation
Department of Applied Chemistry, Yantai University, Yantai 264005, PR China.
Journal Details
This article was published in the following journal.
Name: Journal of the science of food and agriculture
ISSN: 1097-0010
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21997699
- DOI: http://dx.doi.org/10.1002/jsfa.4677
Medical and Biotech [MESH] Definitions
Benzoyl Peroxide
A peroxide derivative that has been used topically for BURNS and as a dermatologic agent in the treatment of ACNE and POISON IVY DERMATITIS. It is used also as a bleach in the food industry.
Triticum
A plant genus of the family POACEAE that is the source of wheat grain. A hybrid with rye (SECALE CEREALE) is called TRITICALE. The seed is ground into FLOUR and used to make BREAD, and is the source of WHEAT GERM AGGLUTININS.
Flour
Ground up seed of WHEAT.
Bromobenzoates
Benzoic acid or benzoic acid esters substituted with one or more bromine atoms.
Nitrobenzoates
Benzoic acid or benzoic acid esters substituted with one or more nitro groups.
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