Genital and Extra-genital Screening for Gonorrhoea using the BD Probetec ET System with an In-house PCR Method Targeting the porA Pseudogene as Confirmatory Test.
Summary of "Genital and Extra-genital Screening for Gonorrhoea using the BD Probetec ET System with an In-house PCR Method Targeting the porA Pseudogene as Confirmatory Test."
Diagnosing gonorrhoea from extra-genital as well as genital sites is important in managing this sexually transmitted disease. In this study we evaluated a screening procedure for Neisseria gonorrhoeae (GC) from all sample sites in a low-prevalence setting. A total of 69,252 specimens submitted for Chlamydia trachomatis testing were also examined for GC on the BD Viper™ platform using the BD Probetec ET system. In order to avoid false-positive results all GC BD reactive samples were re-tested using a PCR method with the porA pseudogene as target. Using this method we screened 170% more samples for GC than in the previous year, in the same population, and diagnosed more than twice as many GC-positive episodes. The BD system can be used successfully to screen extra-genital as well as genital specimen types for GC in a low-prevalence area if it is combined with a validated confirmatory PCR test.
Affiliation
Department of Clinical Microbiology, Hvidovre Hospital, 2650 Hvidovre , Denmark.
Journal Details
This article was published in the following journal.
Name: Acta dermato-venereologica
ISSN: 0001-5555
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22002460
- DOI: http://dx.doi.org/10.2340/00015555-1192
Medical and Biotech [MESH] Definitions
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Paraganglioma, Extra-adrenal
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Neonatal Screening
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Human Papillomavirus 6
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Vagina
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