Phenotypic changes in satellite glial cells in cultured trigeminal ganglia.
Summary of "Phenotypic changes in satellite glial cells in cultured trigeminal ganglia."
Satellite glial cells (SGCs) are specialized cells that form a tight sheath around neurons in sensory ganglia. In recent years, there is increasing interest in SGCs and they have been studied in both intact ganglia and in tissue culture. Here we studied phenotypic changes in SGCs in cultured trigeminal ganglia from adult mice, containing both neurons and SGCs, using phase optics, immunohistochemistry and time-lapse photography. Cultures were followed for up to 14 days. After isolation virtually every sensory neuron is ensheathed by SGCs, as in the intact ganglia. After one day in culture, SGCs begin to migrate away from their parent neurons, but in most cases the neurons still retain an intact glial cover. At later times in culture, there is a massive migration of SGCs away from the neurons and they undergo clear morphological changes, and at 7 days they become spindle-shaped. At one day in culture SGCs express the glial marker glutamine synthetase, and also the purinergic receptor P2X7. From day 2 in culture the glutamine synthetase expression is greatly diminished, whereas that of P2X7 is largely unchanged. We conclude that SGCs retain most of their characteristics for about 24 h after culturing, but undergo major phenotypic changes at later times.
Affiliation
Laboratory of Experimental Surgery, Hadassah-Hebrew University Medical Center, Mount Scopus, Jerusalem 91240, Israel.
Journal Details
This article was published in the following journal.
Name: Neuron glia biology
ISSN: 1741-0533
Pages: 1-7
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22032231
- DOI: http://dx.doi.org/10.1017/S1740925X1100007X
Medical and Biotech [MESH] Definitions
Satellite Cells, Perineuronal
The non-neuronal cells that surround the neuronal cell bodies of the GANGLIA. They are distinguished from the perineuronal satellite oligodendrocytes (OLIGODENDROGLIA) found in the central nervous system.
Glial Fibrillary Acidic Protein
An intermediate filament protein found only in glial cells or cells of glial origin. MW 51,000.
Cells, Cultured
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Satellite Viruses
Defective viruses which can multiply only by association with a helper virus which complements the defective gene. Satellite viruses may be associated with certain plant viruses, animal viruses, or bacteriophages. They differ from satellite RNA; (RNA, SATELLITE) in that satellite viruses encode their own coat protein.
Trigeminal Nerve
The 5th and largest cranial nerve. The trigeminal nerve is a mixed motor and sensory nerve. The larger sensory part forms the ophthalmic, mandibular, and maxillary nerves which carry afferents sensitive to external or internal stimuli from the skin, muscles, and joints of the face and mouth and from the teeth. Most of these fibers originate from cells of the trigeminal ganglion and project to the trigeminal nucleus of the brain stem. The smaller motor part arises from the brain stem trigeminal motor nucleus and innervates the muscles of mastication.
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