Lectin capture strategy for effective analysis of cell secretome.

06:00 EST 9th November 2011 | BioPortfolio

Summary of "Lectin capture strategy for effective analysis of cell secretome."

Secreted proteins play important roles in physiological and pathological processes. However, effective proteomic detection of low abundant secreted proteins is often shielded by the presence of a large amount of intracellular proteins released from unavoidable dead cells during cell culture. In the present work, we applied lectin affinity capture approach to enrich the secreted proteins in the conditioned media (CM) of three human breast cell lines (MCF-10A, MCF-7, MDA-MB-231). Lectin capture showed efficient enrichment of the secreted proteins in CM of all three cell lines and significantly increased the number of secreted proteins detected: from 183 to 292 for MCF-10A, 196 to 325 for MCF-7, and 194 to 368 for MDA-MB-231. Based on more comprehensive profiling of the secreted proteins, we identified 92 secreted proteins which were both up-regulated in MCF-7 and MDA-MB-231, with 82 only found in lectin captured samples. It should be noted that among these 82 potential biomarkers, 59 were not reported in previous proteomic studies of breast cancer. These data indicate that the lectin capture approach is a powerful means to move toward more comprehensive analysis and comparison of secretomes.


School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.

Journal Details

This article was published in the following journal.

Name: Proteomics
ISSN: 1615-9861


PubMed Articles [40388 Associated PubMed Articles listed on BioPortfolio]

Use of Composite Protein Database including Search Result Sequences for Mass Spectrometric Analysis of Cell Secretome.

Mass spectrometric (MS) data of human cell secretomes are usually run through the conventional human database for identification. However, the search may result in false identifications due to contami...

Lectin Engineering, a Molecular Evolutionary Approach to Expanding the Lectin Utilities.

In the post genomic era, glycomics-the systematic study of all glycan structures of a given cell or organism-has emerged as an indispensable technology in various fields of biology and medicine. Lecti...

Lipopolysaccharide preconditioning of adipose-derived stem cells improves liver-regenerating activity of the secretome.

Growing recognition of paracrine mechanisms in stem cell plasticity has resulted in considerable interest in stem cell-derived secretome. The aim of this study was to investigate the effects of lipopo...

Plant secretomics.

Plant secretomes are the proteins secreted by the plant cells and are involved in the maintenance of cell wall structure, relationship between host and pathogen, communication between different cells ...

Effect of Chum Salmon Egg Lectin on Tight Junctions in Caco-2 Cell Monolayers.

The effect of a chum salmon egg lectin (CSL3) on tight junction (TJ) of Caco-2 cell monolayers was investigated. The lectin opened TJ as indicated by the decrease of the transepithelial electrical res...

Clinical Trials [7030 Associated Clinical Trials listed on BioPortfolio]

Mistletoe Lectin in Treating Patients With Advanced Solid Tumors That Have Not Responded to Previous Therapy

RATIONALE: Mistletoe lectin may slow the growth of cancer cells and be an effective treatment for solid tumors. PURPOSE: Phase I trial to study the effectiveness of mistletoe lectin in tr...

The Human Blastocyst Secretome and Implantome

The aim of this study is to identify the protein secretion and consumption profile of the human blastocyst (secretome) and the implanted blastocyst (implantome) using the protein array tec...

Study on Mannan Binding Lectin (MBL) Substitution in MBL-Deficient Children With Chemotherapy-Induced Neutropenia

The pharmacokinetics, and clinical and biological effects of MBL replacement therapy in MBL-deficient children during chemotherapy-induced neutropenia were studied.

Boron Neutron Capture Therapy in Treating Patients With Melanoma

RATIONALE: Boron neutron capture therapy may selectively kill tumor cells without harming normal tissue. PURPOSE: Phase II trial to study the effectiveness of boron neutron capture therap...

Preventing Fucose-dependent Binding of Aspergillus and Pseudomonas to Lung Mucin

The investigators will collect samples of sputum from healthy volunteers and patients with cystic fibrosis for the purpose of: a) purifying airway mucins for plate-based binding studies an...

Medical and Biotech [MESH] Definitions

Strategy for the analysis of RANDOMIZED CONTROLLED TRIALS AS TOPIC that compares patients in the groups to which they were originally randomly assigned.

A subclass of NK cell lectin-like receptors that associates with a variety of members of NK CELL LECTIN-LIKE RECEPTOR SUBFAMILY C to form heterodimeric receptors for HLA-E antigen.

A subclass of NK cell lectin-like receptors that associates with members of NK CELL LECTIN-LIKE RECEPTOR SUBFAMILY D to form heterodimeric receptors for HLA-E antigen.

A subclass of NK cell lectin-like receptors that includes both inhibitory and stimulatory members.

An inhibitory subclass of NK cell lectin-like receptors that interacts with CLASS I MAJOR HISTOCOMPATIBILITY ANTIGENS and prevents the activation of NK CELLS.


Relevant Topic

Breast Cancer
Track and monitor developments in breast cancer research and commercial development.  Follow the tabs above to read the latest global news, research, clinical trials on breast cancer and follow companies active in the development of breast cancer tr...

Searches Linking to this Article