Simple and rapid liquid chromatography method for determination of moxifloxacin in saliva.
Summary of "Simple and rapid liquid chromatography method for determination of moxifloxacin in saliva."
A high performance liquid chromatographic method for determination of moxifloxacin in human saliva was developed. The method involved deproteinisation of the sample with perchloric acid and analysis of the supernatant using a reversed-phase C18 column (150mm) and fluorescence detection at an excitation wavelength of 290nm and an emission wavelength of 460nm. The assay was specific for moxifloxacin and linear from 0.25 to 10.0μg/ml. The relative standard deviation of intra- and inter-day assays was lower than 10%. The average recovery of moxifloxacin from saliva was 101%. Due to its simplicity, the assay can be used for pharmacokinetic studies of moxifloxacin.
Affiliation
Tuberculosis Research Centre (Indian Council of Medical Research), Chetput, Chennai, India.
Journal Details
This article was published in the following journal.
Name: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN: 1873-376X
Pages: 3663-7
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22024393
- DOI: http://dx.doi.org/10.1016/j.jchromb.2011.09.047
Medical and Biotech [MESH] Definitions
Countercurrent Distribution
A method of separation of two or more substances by repeated distribution between two immiscible liquid phases that move past each other in opposite directions. It is a form of liquid-liquid chromatography. (Stedman, 25th ed)
Chromatography, Gas
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
Chromatography, Liquid
Chromatographic techniques in which the mobile phase is a liquid.
Chromatography, Agarose
A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million.
Solid Phase Extraction
An extraction method that separates analytes using a solid phase and a liquid phase. It is used for preparative sample cleanup before analysis by CHROMATOGRAPHY and other analytical methods.
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