Determination of pinostrobin in rat plasma by LC-MS/MS: application to pharmacokinetics.
Summary of "Determination of pinostrobin in rat plasma by LC-MS/MS: application to pharmacokinetics."
A rapid and sensitive method for the determination of pinostrobin in rat plasma was developed using liquid chromatography tandem mass spectrometry (LC-MS/MS) for the first time. Isoliquiritigenin was used as an internal standard in rat plasma. Chromatographic separation was performed on an HiQ Sil C(18) column with isocratic elution at a flow rate of 1mL/min. The mobile phase consisted of water and methanol (9:91, v/v) containing 0.1% formic acid. The quantification limit was 10ng/mL within a linear range of 10-1000ng/mL (R=0.9984). The intra- and inter-day assay precision ranged from 3.8-5.3% to 3.2-5.2%, respectively, and the intra- and inter-day assay accuracy was between 93.2-95.1% and 95.5-104.3%, respectively. Our results indicated that the LC-MS/MS method is effective for pharmacokinetic study of pinostrobin in rat plasma.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
This article was published in the following journal.
Name: Journal of pharmaceutical and biomedical analysis
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/21840667
- DOI: http://dx.doi.org/10.1016/j.jpba.2011.07.038
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