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Backgroundâ€‚ A role for the innate immune system in driving the autoimmune T cell cascade in psoriasis has been proposed. Toll-like receptors-(TLR)-2 and -4 play a role in inflammation, atherosclerosis, and their specific role in psoriasis remains unclear. Objectiveâ€‚ To evaluate TLR2 and TLR4 gene expression levels in peripheral blood mononuclear cells from psoriatic patients. Methodsâ€‚ Changes in TLR2/4 gene expressions were evaluated using quantitative real-time reverse transcription polymerase chain reaction in peripheral blood mononuclear cells, from twenty-one patients with severe psoriasis, and analysed whether there was any correlation with cytokine plasma levels (T-helper 0-, T-helper 1-, T-helper 2- or regulatory T cells-type), or Calprotectin and with S100A8 and S100A9 gene expression levels. Eleven non-psoriatic healthy controls were analysed. Resultsâ€‚ A clear increase in TLR4 gene expression was observed (3.84â€ƒÂ±â€ƒ0.93, nâ€ƒ=â€ƒ21) together with a moderate increase in TLR2 expression (1.522â€ƒÂ±â€ƒ0.31, nâ€ƒ=â€ƒ21). Both TLR4 and TLR2 gene expressions were significantly augmented in psoriatic patients compared with controls (all Pâ€ƒ<â€ƒ0.001). Correlations between TLR2 and S100A9 gene expressions (râ€ƒ=â€ƒ0.5145, Pâ€ƒ=â€ƒ0.0170, nâ€ƒ=â€ƒ21); and between TLR2 expression and plasma interleukin-2 (râ€ƒ=â€ƒ0.5667, Pâ€ƒ=â€ƒ0.0074); interleukin-4 (râ€ƒ=â€ƒ0.4766, Pâ€ƒ=â€ƒ0.0289), interleukin-10 (râ€ƒ=â€ƒ0.4355, Pâ€ƒ=â€ƒ0.0484) and interleukin-13 (râ€ƒ=â€ƒ0.4603, Pâ€ƒ=â€ƒ0.0358), were found. When patients with atheroma plaque were considered (nâ€ƒ=â€ƒ7), both TLR4 (3.47â€ƒÂ±â€ƒ0.99, Pâ€ƒ=â€ƒ0.0156) and TLR2 (1.63â€ƒÂ±â€ƒ0.31, Pâ€ƒ=â€ƒ0.0156) expressions were significantly increased vs. controls and correlated with plasma TNF-Î± (râ€ƒ=â€ƒ0.8929, Pâ€ƒ=â€ƒ0.0123, in both cases). Conclusionâ€‚ Differential TLR4/2 gene expressions on psoriatic peripheral blood mononuclear cells and correlations with regulatory and/or proinflammatory cytokines and/or damage-associated molecular pattern molecule S100A9 emphasize innate immune response role in psoriasis.
Department of Cellular Biology and Immunology, Instituto de ParasitologÃa y Biomedicina LÃ³pez-Neyra, IPBLN-CSIC, P.T. Ciencias de la Salud, Armilla, Granada, Spain Department of Dermatology Department of Radiology, San Cecilio Un
This article was published in the following journal.
Name: Journal of the European Academy of Dermatology and Venereology : JEADV
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