Increased gene expression of Toll-like receptor 4 on peripheral blood mononuclear cells in patients with psoriasis.
Summary of "Increased gene expression of Toll-like receptor 4 on peripheral blood mononuclear cells in patients with psoriasis."
Backgroundâ€‚ A role for the innate immune system in driving the autoimmune T cell cascade in psoriasis has been proposed. Toll-like receptors-(TLR)-2 and -4 play a role in inflammation, atherosclerosis, and their specific role in psoriasis remains unclear. Objectiveâ€‚ To evaluate TLR2 and TLR4 gene expression levels in peripheral blood mononuclear cells from psoriatic patients. Methodsâ€‚ Changes in TLR2/4 gene expressions were evaluated using quantitative real-time reverse transcription polymerase chain reaction in peripheral blood mononuclear cells, from twenty-one patients with severe psoriasis, and analysed whether there was any correlation with cytokine plasma levels (T-helper 0-, T-helper 1-, T-helper 2- or regulatory T cells-type), or Calprotectin and with S100A8 and S100A9 gene expression levels. Eleven non-psoriatic healthy controls were analysed. Resultsâ€‚ A clear increase in TLR4 gene expression was observed (3.84â€ƒÂ±â€ƒ0.93, nâ€ƒ=â€ƒ21) together with a moderate increase in TLR2 expression (1.522â€ƒÂ±â€ƒ0.31, nâ€ƒ=â€ƒ21). Both TLR4 and TLR2 gene expressions were significantly augmented in psoriatic patients compared with controls (all Pâ€ƒ<â€ƒ0.001). Correlations between TLR2 and S100A9 gene expressions (râ€ƒ=â€ƒ0.5145, Pâ€ƒ=â€ƒ0.0170, nâ€ƒ=â€ƒ21); and between TLR2 expression and plasma interleukin-2 (râ€ƒ=â€ƒ0.5667, Pâ€ƒ=â€ƒ0.0074); interleukin-4 (râ€ƒ=â€ƒ0.4766, Pâ€ƒ=â€ƒ0.0289), interleukin-10 (râ€ƒ=â€ƒ0.4355, Pâ€ƒ=â€ƒ0.0484) and interleukin-13 (râ€ƒ=â€ƒ0.4603, Pâ€ƒ=â€ƒ0.0358), were found. When patients with atheroma plaque were considered (nâ€ƒ=â€ƒ7), both TLR4 (3.47â€ƒÂ±â€ƒ0.99, Pâ€ƒ=â€ƒ0.0156) and TLR2 (1.63â€ƒÂ±â€ƒ0.31, Pâ€ƒ=â€ƒ0.0156) expressions were significantly increased vs. controls and correlated with plasma TNF-Î± (râ€ƒ=â€ƒ0.8929, Pâ€ƒ=â€ƒ0.0123, in both cases). Conclusionâ€‚ Differential TLR4/2 gene expressions on psoriatic peripheral blood mononuclear cells and correlations with regulatory and/or proinflammatory cytokines and/or damage-associated molecular pattern molecule S100A9 emphasize innate immune response role in psoriasis.
Department of Cellular Biology and Immunology, Instituto de ParasitologÃa y Biomedicina LÃ³pez-Neyra, IPBLN-CSIC, P.T. Ciencias de la Salud, Armilla, Granada, Spain Department of Dermatology Department of Radiology, San Cecilio Un
This article was published in the following journal.
Name: Journal of the European Academy of Dermatology and Venereology : JEADV
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22142593
- DOI: http://dx.doi.org/10.1111/j.1468-3083.2011.04372.x
[This corrects the article on p. e62870 in vol. 8.].
To better understand the role of downstream Toll-like receptor (TLR) signaling during acute cerebral ischemia, we performed cDNA microarrays, on brain RNA, and cytokine arrays, on serum, from wild typ...
The line between vascular dementia (VaD) and Alzheimer's disease (AD) is often blurred. In this study we investigated whether adenosine A2A receptor (A2AR) expression can be used to differentiate betw...
A composite, peripheral gene expression score based on quantitative RNA-measurements has been validated for detecting stenosis against invasive coronary X-ray angiography. IVUS/VH has been validated f...
Eosinophils are inflammatory cells of particular relevance to asthma exacerbations. Neuropeptide S (NPS) receptor was identified in a search for asthma susceptibility genes, where the risk haplotypes...
The purpose of this study is to determine whether asthmatics have different adenosine receptor expression profiles than healthy controls. We hypothesize that asthmatics will have increased...
Regular exercise is known to produce significant health benefits and to reduce the risk of heart diseases, although how this benefit occurs is not well understood. White blood cells are kn...
The purpose of the proposed pilot study is to identify factors which are associated with periods of high ventricular arrhythmia burden. This will be performed by analysis of gene expressi...
The first objective of this study is to determine if increased expression of one or more members of the toll-like receptor (TLR) family of receptors that are found on inflammatory cells (p...
To validate the use of Corusâ„¢ CAD blood assay in subjects who are referred for the work-up of coronary artery disease. The study will evaluate the clinical utility of a gene expression t...
Medical and Biotech [MESH] Definitions
A pattern recognition receptor that forms heterodimers with TOLL-LIKE RECEPTOR 2.
A pattern recognition receptor that forms heterodimers with other TOLL-LIKE RECEPTORS. It interacts with multiple ligands including PEPTIDOGLYCAN, bacterial LIPOPROTEINS, lipoarabinomannan, and a variety of PORINS.
A family of pattern recognition receptors characterized by an extracellular leucine-rich domain and a cytoplasmic domain that share homology with the INTERLEUKIN 1 RECEPTOR and the DROSOPHILA toll protein. Following pathogen recognition, toll-like receptors recruit and activate a variety of SIGNAL TRANSDUCING ADAPTOR PROTEINS.
A form of gene interaction whereby the expression of one gene interferes with or masks the expression of a different gene or genes. Genes whose expression interferes with or masks the effects of other genes are said to be epistatic to the effected genes. Genes whose expression is affected (blocked or masked) are hypostatic to the interfering genes.
A pattern recognition receptor that forms heterodimers with TLR2 RECEPTOR. The complex interacts with a variety of ligands including LIPOPROTEINS from MYCOPLASMA.