Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria.
Summary of "Disposable roll-to-roll hot embossed electrophoresis chip for detection of antibiotic resistance gene mecA in bacteria."
We present a high-throughput roll-to-roll (R2R) manufacturing process for foil-based polymethyl methacrylate (PMMA) chips of excellent optical quality. These disposable, R2R hot embossed microfluidic chips are used for the identification of the antibiotic resistance gene mecA in Staphylococcus epidermidis. R2R hot embossing is an emerging manufacturing technology for polymer microfluidic devices. It is based on continuous feeding of a thermoplastic foil through a pressurized area between a heated embossing cylinder and a blank counter cylinder. Although mass fabrication of foil-based microfluidic chips and their use for biological applications were foreseen already some years ago, no such studies have been published previously.
Affiliation
VTT Technical Research Centre of Finland, Kaitoväylä 1, P.O. Box 1100, 90571, Oulu, Finland. ralph.liedert@vtt.fi.
Journal Details
This article was published in the following journal.
Name: Lab on a chip
ISSN: 1473-0189
Pages: 333-9
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22127494
- DOI: http://dx.doi.org/10.1039/c1lc20782b
Medical and Biotech [MESH] Definitions
Comet Assay
A genotoxicological technique for measuring DNA damage in an individual cell using single-cell gel electrophoresis. Cell DNA fragments assume a "comet with tail" formation on electrophoresis and are detected with an image analysis system. Alkaline assay conditions facilitate sensitive detection of single-strand damage.
Electrophoresis, Gel, Two-dimensional
Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.
Substance Abuse Detection
Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.
Electrophoresis, Microchip
A highly miniaturized version of ELECTROPHORESIS performed in a microfluidic device.
Disposable Equipment
Apparatus, devices, or supplies intended for one-time or temporary use.
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