Simultaneous determination of codeine, ephedrine, guaiphenesin and chlorpheniramine in beagle dog plasma using high performance liquid chromatography coupled with tandem mass spectrometric detection: Application to a bioequivalence study.
Summary of "Simultaneous determination of codeine, ephedrine, guaiphenesin and chlorpheniramine in beagle dog plasma using high performance liquid chromatography coupled with tandem mass spectrometric detection: Application to a bioequivalence study."
A rapid and sensitive method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of codeine, ephedrine, guaiphenesin and chlorpheniramine in beagle dog plasma has been developed and validated. Following liquid-liquid extraction, the analytes were separated on a reversed-phase C(18) column (150mm×2.0mm, 3μm) using formic acid:10mM ammonium acetate:methanol (0.2:62:38, v/v/v) as mobile phase at a flow rate of 0.2mL/min and analyzed by a triple-quadrupole mass spectrometer in the selected reaction monitoring (SRM) mode. The method was linear for all analytes over the following concentration (ng/mL) ranges: codeine 0.08-16; ephedrine 0.8-160; guaiphenesin 80-16,000; chlorpheniramine 0.2-40. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. It is the first time that the validated HPLC-MS/MS method was successfully applied to a bioequivalence study in 6 healthy beagle dogs.
Affiliation
Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing 210009, China; Center for Instrumental Analysis, China Pharmaceutical University, Nanjing 210009, China.
Journal Details
This article was published in the following journal.
Name: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN: 1873-376X
Pages: 3937-42
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22119507
- DOI: http://dx.doi.org/10.1016/j.jchromb.2011.11.001
Medical and Biotech [MESH] Definitions
Oxycodone
Semisynthetic derivative of CODEINE that acts as a narcotic analgesic more potent and addicting than codeine.
Heart-lung Transplantation
The simultaneous, or near simultaneous, transference of heart and lungs from one human or animal to another.
Plasma Exchange
Removal of plasma and replacement with various fluids, e.g., fresh frozen plasma, plasma protein fractions (PPF), albumin preparations, dextran solutions, saline. Used in treatment of autoimmune diseases, immune complex diseases, diseases of excess plasma factors, and other conditions.
Anaerobiospirillum
A genus of gram-negative, spiral-shaped bacteria in the family SUCCINIVIBRIONACEAE. It has been isolated from the THROAT and COLON of beagle dogs and from human FECES.
Chlorpheniramine
A histamine H1 antagonist used in allergic reactions, hay fever, rhinitis, urticaria, and asthma. It has also been used in veterinary applications. One of the most widely used of the classical antihistaminics, it generally causes less drowsiness and sedation than PROMETHAZINE.
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