Musashi 1 positive cells derived from mouse embryonic stem cells can differentiate into neural and intestinal epithelial-like cells in vivo.
Summary of "Musashi 1 positive cells derived from mouse embryonic stem cells can differentiate into neural and intestinal epithelial-like cells in vivo."
Background: Musashi 1 (Msi1) is regarded as a marker for neural and intestinal epithelial stem cells. However, it's still unclear whether Msi1 positive cells derived from mouse embryonic stem cells have the ability to differentiate into neural or intestinal epithelial cells. Methods: To reveal the potential of Msi1 positive cells derived from embryonic stem cells, Msi1 expression was detected during the development of embryonic stem cells in vitro. A pMsi1-GFP reporter plasmid was constructed in order to sort Msi1 positive cells from the differentiated cell population. The GFP positive cells (i.e., Msi1 positive cells) were sorted by fluorescence activated cell sorting and were hypodermically engrafted into the backs of NOD/SCID mice. The presence of neural and intestinal epithelial cells was determined in the grafts from Msi1 positive cells. Results: The strongest expression of Msi1 was detected on the 11th day of differentiation. Msi1 within the GFP positive cells was highly expressed after the transfection of the pMsi1-GFP vector in vitro. The markers for neural cells (Nestin and Tubulin) and intestinal epithelial cells (Fatty acid binding protein 2, Lysozyme and Chromogranin A) were highly expressed in the grafts from Msi1-positive cells relative to that of the Msi1-negative group (P<0.05). The grafts from the Msi1-negative cells contained more mesodermal tissues. Conclusions: The pMsi1-GFP vector can be used to sort Msi1 positive cells from a cell population derived from embryonic stem cells. The Msi1-positive cells can differentiate into neural and intestinal epithelial-like cells in vivo.
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Journal Details
This article was published in the following journal.
Name: Cell biology international
ISSN: 1095-8355
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20670215
- DOI: http://dx.doi.org/10.1042/CBI20100108
Medical and Biotech [MESH] Definitions
Embryonic Stem Cells
Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.
Embryonal Carcinoma Stem Cells
The malignant stem cells of TERATOCARCINOMAS, which resemble pluripotent stem cells of the BLASTOCYST INNER CELL MASS. The EC cells can be grown in vitro, and experimentally induced to differentiate. They are used as a model system for studying early embryonic cell differentiation.
Induced Pluripotent Stem Cells
Cells from adult organisms that have been reprogrammed into a pluripotential state similar to that of EMBRYONIC STEM CELLS.
Stage-specific Embryonic Antigens
Cell-surface molecules that exhibit lineage-restricted patterns of expression during EMBRYONIC DEVELOPMENT. The antigens are useful markers in the identification of EMBRYONIC STEM CELLS.
Myeloid Progenitor Cells
Stem cells derived from HEMATOPOIETIC STEM CELLS. Derived from these myeloid progenitor cells are the MEGAKARYOCYTES; ERYTHROID CELLS; MYELOID CELLS; and some DENDRITIC CELLS.
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