Polyamines analysis by HPLC and their application as tumor markers.
Summary of "Polyamines analysis by HPLC and their application as tumor markers."
Cancers are one of the main causes of human deaths globally. Great effort has been dedicated to the search for sensitive and specific markers of cancer. Polyamines including mainly putrescine (PUT), spermidine (SPD), and spermine (SP), are promising tumor markers since their excretion is frequently elevated in patients with various types of cancers. In the present study, we developed an efficient high performance liquid chromatographic (HPLC) method for the determination of polyamines in human serum using dansyl chloride for pre-column derivatization. All polyamines were separated within 10 min. The analytical method is simple, rapid, and highly reproducible. We applied of 11 cancers as objects of study, and made comparisons between polyamines and other 24 common tumor markers with six indexes: specificity, sensitivity, negative predictive value, positive predictive value, total effective, and the mean value. We drew the conclusion that polyamines are promising tumor markers, and they might be of great value in diagnosing cancers, predicting therapeutic success, or indicating the relapse of tumors.
Affiliation
Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China.
Journal Details
This article was published in the following journal.
Name: Frontiers in bioscience (Elite edition)
ISSN: 1945-0508
Pages: 1795-801
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22201995
- DOI: http://dx.doi.org/
Medical and Biotech [MESH] Definitions
Tumor Markers, Biological
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
Neoplasm Proteins
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (TUMOR MARKERS, BIOLOGICAL) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Genome-wide Association Study
An analysis comparing the allele frequencies of all available (or a whole GENOME representative set of) polymorphic markers in unrelated patients with a specific symptom or disease condition, and those of healthy controls to identify markers associated with a specific disease or condition.
Biogenic Polyamines
Biogenic amines having more than one amine group. These are long-chain aliphatic compounds that contain multiple amino and/or imino groups. Because of the linear arrangement of positive charge on these molecules, polyamines bind electrostatically to ribosomes, DNA, and RNA.
Antigens, Tumor-associated, Carbohydrate
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
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