Analysis of proteomic changes in roots of soybean seedlings during recovery after flooding.
Summary of "Analysis of proteomic changes in roots of soybean seedlings during recovery after flooding."
A proteomic approach was used to identify proteins involved in post-flooding recovery in soybean roots. Two-day-old soybean seedlings were flooded with water for up to 3days. After the flooding treatment, seedlings were grown until 7days after sowing and root proteins were then extracted and separated using two-dimensional polyacrylamide gel electrophoresis (2-DE). Comparative analysis of 2-D gels of control and 3day flooding-experienced soybean root samples revealed 70 differentially expressed protein spots, from which 80 proteins were identified. Many of the differentially expressed proteins are involved in protein destination/storage and metabolic processes. Clustering analysis based on the expression profiles of the 70 differentially expressed protein spots revealed that 3days of flooding causes significant changes in protein expression, even during post-flooding recovery. Three days of flooding resulted in downregulation of ion transport-related proteins and upregulation of proteins involved in cytoskeletal reorganization, cell expansion, and programmed cell death. Furthermore, 7 proteins involved in cell wall modification and S-adenosylmethionine synthesis were identified in roots from seedlings recovering from 1day of flooding. These results suggest that alteration of cell structure through changes in cell wall metabolism and cytoskeletal organization may be involved in post-flooding recovery processes in soybean seedlings.
Affiliation
National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan; Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Karaj 31587-77871, Iran.
Journal Details
This article was published in the following journal.
Name: Journal of proteomics
ISSN: 1876-7737
Pages: 878-93
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22037232
- DOI: http://dx.doi.org/10.1016/j.jprot.2011.10.002
Medical and Biotech [MESH] Definitions
Soybean Oil
Oil from soybean or soybean plant.
Rhizome
Root-like underground horizontal stem of plants that produces shoots above and roots below. Distinguished from true roots which don't have buds and nodes. Similar to true roots in being underground and thickened by storage deposits.
Activation Analysis
A method of chemical analysis based on the detection of characteristic radionuclides following a nuclear bombardment. It is also known as radioactivity analysis. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Spinal Nerve Roots
The paired bundles of nerve fibers entering and leaving the spinal cord at each segment. The dorsal and ventral nerve roots join to form the mixed segmental spinal nerves. The dorsal roots are generally afferent, formed by the central projections of the spinal (dorsal root) ganglia sensory cells, and the ventral roots efferent, comprising the axons of spinal motor and autonomic preganglionic neurons. There are, however, some exceptions to this afferent/efferent rule.
Plant Shoots
New immature growth of a plant including stem, leaves, tips of branches, and SEEDLINGS.
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