BVPaP-3, a T7-Like Lytic Phage of Pseudomonas aeruginosa: Its Isolation and Characterisation.
Summary of "BVPaP-3, a T7-Like Lytic Phage of Pseudomonas aeruginosa: Its Isolation and Characterisation."
The increasing emergence of antibiotic-resistant bacteria has produced a growing interest among scientists in bacteriophages as alternative antimicrobial agents. This article reports a lytic phage against an antibiotic-resistant strain of Pseudomonas aeruginosa. Phage BVPaP-3 is a member of the Podoviridae family and morphologically similar to the T7-like phage gh-1. The phage has a hexagonal head of 58-59 nm in diameter and a short tail of 10 × 8 nm. It is stable at a wide range of pH (6-10) and temperatures (4-40°C). Its optimal growth temperature is 37°C and the adsorption rate constant is 1.19 × 10(-9). Latent and eclipse periods are 20 and 15 min, respectively, and the burst size is 44 after 35 min at 37°C. The phage has a DNA size of 41.31 kb and a proteome of 11 proteins. The major protein is 33 kDa in size.
Affiliation
Department of Environmental Science, University of Pune, Pune, India.
Journal Details
This article was published in the following journal.
Name: Current microbiology
ISSN: 1432-0991
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22215482
- DOI: http://dx.doi.org/10.1007/s00284-011-0071-6
Medical and Biotech [MESH] Definitions
Pseudomonas Phages
Viruses whose host is Pseudomonas. A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6.
Pseudomonas Aeruginosa
A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.
Rna Phages
Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.
Pyocyanine
Antibiotic pigment produced by Pseudomonas aeruginosa.
Bacteriophage Pf1
A species of filamentous Pseudomonas phage in the genus INOVIRUS, family INOVIRIDAE.
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