Construction of an infectious cDNA clone of Culex flavivirus, an insect-specific flavivirus from Culex mosquitoes.
Summary of "Construction of an infectious cDNA clone of Culex flavivirus, an insect-specific flavivirus from Culex mosquitoes."
Culex flavivirus (CxFV) is an insect-specific flavivirus that has recently been detected in various Culex spp. mosquitoes worldwide. Here, we report the successful construction of a full-length infectious cDNA clone of a Tokyo strain, CxFV-NIID21. The full-length CxFV-NIID21 cDNA was cloned into the low-copy-number plasmid pMW119, which was stably amplified in Escherichia coli. Transfection of a mosquito cell line with in vitro-transcribed RNA from the cDNA clone resulted in the production of recombinant progeny virus with growth properties, cytopathogenicity, and virion morphology similar to the parental virus.
Affiliation
Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan, hisawa@nih.go.jp.
Journal Details
This article was published in the following journal.
Name: Archives of virology
ISSN: 1432-8798
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22297417
- DOI: http://dx.doi.org/10.1007/s00705-012-1240-z
Medical and Biotech [MESH] Definitions
Reverse Transcriptase Polymerase Chain Reaction
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Clone Cells
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Mosquito Nets
Free-standing or supported light weight meshwork fabric made of cotton, silk, polyester or other material having openings too small to allow entry of mosquitoes or other insects, thereby protecting against INSECT BITES; INSECT STINGS, and insect-borne diseases.
Flavivirus Infections
Infections with viruses of the genus FLAVIVIRUS, family FLAVIVIRIDAE.
Financing, Construction
Funding resources and procedures for capital improvement or the construction of facilities.
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