Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design.
Summary of "Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design."
Aptamer-based fluorescence detection of platelet-derived growth factor (PDGF) on a functionalized diamond surface was demonstrated. In this work, a sandwich design based on the ability of PDGF to bind with aptamers at its two available binding sites was employed. It was found that this sandwich design approach significantly increases the fluorescence signal intensity, and thereby a very low detection limit of 4 pM was achieved. The effect of the ionic strength of MgCl(2) buffer solution was also investigated, and the most favourable binding for PDGF-BB occurred at a Mg(2+) concentration of 5.5 mM. Since the aptamers bind to the target PDGF with high affinity, fluorescence detection exhibited high selectivity towards different biomolecules. The high reproducibility of detection was confirmed by performing three cycles of measurements over a period of three days.
Affiliation
School of Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo 169-8555, Japan. ruslinda@toki.waseda.jp.
Journal Details
This article was published in the following journal.
Name: The Analyst
ISSN: 1364-5528
Pages: 1692-7
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22349046
- DOI: http://dx.doi.org/10.1039/c2an15933c
Medical and Biotech [MESH] Definitions
Receptors, Platelet-derived Growth Factor
Specific receptors on cell membranes that react with PLATELET-DERIVED GROWTH FACTOR, its analogs, or antagonists. The alpha PDGF receptor (RECEPTOR, PLATELET-DERIVED GROWTH FACTOR ALPHA) and the beta PDGF receptor (RECEPTOR, PLATELET-DERIVED GROWTH FACTOR BETA) are the two principle types of PDGF receptors. Activation of the protein-tyrosine kinase activity of the receptors occurs by ligand-induced dimerization or heterodimerization of PDGF receptor types.
Genes, Sis
Retrovirus-associated DNA sequences (v-sis) originally isolated from the simian sarcoma virus (SSV). The proto-oncogene c-sis codes for a growth factor which is the B chain of PLATELET-DERIVED GROWTH FACTOR. v-sis or overexpression of c-sis causes tumorigenesis. The human sis gene is located at 22q12.3-13.1 on the long arm of chromosome 22.
Grb2 Adaptor Protein
A signal transducing adaptor protein that links extracellular signals to the MAP KINASE SIGNALING SYSTEM. Grb2 associates with activated EPIDERMAL GROWTH FACTOR RECEPTOR and PLATELET-DERIVED GROWTH FACTOR RECEPTORS via its SH2 DOMAIN. It also binds to and translocates the SON OF SEVENLESS PROTEINS through its SH3 DOMAINS to activate PROTO-ONCOGENE PROTEIN P21(RAS).
Platelet-derived Growth Factor
Mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication.
Proto-oncogene Proteins C-sis
Cellular DNA-binding proteins encoded by the sis gene (GENES, SIS). c-sis proteins make up the B chain of PLATELET-DERIVED GROWTH FACTOR. Overexpression of c-sis causes tumorigenesis.
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