Crystallography on a chip.
Summary of "Crystallography on a chip."
A new chip-based crystal-mounting approach for rapid room-temperature data collection from numerous crystals is described. This work was motivated by the recent development of X-ray free-electron lasers. These novel sources deliver very intense femtosecond X-ray pulses that promise to yield high-resolution diffraction data of nanocrystals before their destruction by radiation damage. Thus, the concept of `diffraction before destruction' requires rapid replenishment of the sample for each exposure. The chip promotes the self-assembly of an array of protein crystals on a surface. Rough features on the surface cause the crystals to adopt random orientations, allowing efficient sampling of reciprocal space.
Affiliation
Max Planck Research Group for Structural Dynamics, Center for Free-Electron Laser Science, Department of Physics, University of Hamburg, Notkestrasse 85, 22607 Hamburg, Germany.
Journal Details
This article was published in the following journal.
Name: Acta crystallographica. Section D, Biological crystallography
ISSN: 1399-0047
Pages: 321-3
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22349234
- DOI: http://dx.doi.org/10.1107/S0907444911055296
Medical and Biotech [MESH] Definitions
Crystallography, X-ray
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Lab-on-a-chip Devices
Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves.
Crystallography
The branch of science that deals with the geometric description of crystals and their internal arrangement. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Neutron Diffraction
The scattering of NEUTRONS by matter, especially crystals, with accompanying variation in intensity due to interference effects. It is useful in CRYSTALLOGRAPHY and POWDER DIFFRACTION.
X-ray Diffraction
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
PubMed Articles
A little more than 10 years ago Andreas Manz and Harp Minhas launched the Lab on a Chip journal. Their adventurous initiative has developed into a very successful and high-ranked journal, now establis...
Gjønnes Medal in Electron Crystallography - call for nominations.
Nominations for the Gjønnes Medal in Electron Crystallography are requested.
Lab-on-a-chip technologies for single-molecule studies.
Recent developments on various lab-on-a-chip techniques allow miniaturized and integrated devices to perform on-chip single-molecule studies. Fluidic-based platforms that utilize unique microscale flu...
High-throughput immunoassay through in-channel microfluidic patterning.
We have developed an integrated microfluidic immunoassay chip for high-throughput sandwich immunoassay tests. The chip creates an array of reactive patterns through mechanical protection by actuating...
Clinical Trials
The purpose of the study is to assess the efficacy and the safety of Chlorhexidine Gluconate chip (Periochip®) versus Placebo Chip in treatment of symptoms in patients with Peri-Implantit...
DNA Chip Based Prognosis of Lung Cancer
The purpose of the study is to determine whether a DNA chip, designed by Medical Prognosis Institute (MPI), can provide an accurate prognosis for survival of NSCLC (adeno-, squamous and la...
The purpose of this study is to determine the effect of the placement of a PerioChip Plus (flurbiprofen/chlorhexidine - FBP/CHX) formulation versus PerioChip (chlorhexidine) formulation ve...
Genetic Studies in Liver Cancer Patients
Hepatocellular carcinoma (HCC) is one the most common malignancies in the world, especially in sub-Saharan Africa and Southeast Asia. Since 1984, it has been the leading cause of cancer de...
The aim of the study is to explore the possibility to identify, at an early stage after a renal graft and from blood samples collected within first months after graft, a predictive transcr...
