Three-phase hollow fiber liquid phase microextraction of warfarin from human plasma and its determination by high-performance liquid chromatography.
Summary of "Three-phase hollow fiber liquid phase microextraction of warfarin from human plasma and its determination by high-performance liquid chromatography."
A simple, inexpensive and efficient sample preparation technique, three-phase hollow fiber liquid phase microextraction (HF-LPME), followed by high-performance liquid chromatography-ultra violet detection (HPLC-UV) was used for the analysis of warfarin in human plasma. Warfarin was extracted from 11.0 ml of aqueous solution with pH=2.3 (donor phase) into 1-octanol immobilized in the wall pores of a porous hollow fiber and then extracted into the acceptor phase with pH=11.0 located in the lumen of the hollow fiber. After the extraction, the acceptor phase was directly injected into the HPLC system for quantification. Different factors affecting the HF-LPME including nature of organic extraction solvent, pH of donor and acceptor phases, stirring rate, extraction time and salt addition to the sample solution (donor phase) were investigated and optimized. Under the optimized conditions (1-octanol as organic solvent, pH(donor)=2.3, pH(acceptor)=11.0, stirring speed of 1000 rpm, extraction time of 30 min, without addition of salt), limit of detection (LOD) of 5 ng ml(-1) and enrichment factor of 225 were obtained. The calibration curve was linear within the range of 15-550 ng ml(-1) with the square of correlation coefficient of 0.9984. The values of intra-day relative standard deviation (RSD) and inter-day RSD were 4.2% and 11.1%, respectively. The method was applied successfully for the analysis of warfarin in plasma sample from a patient under treatment with this drug and excellent sample clean-up was observed.
Affiliation
Department of Chemistry, University of Mazandaran, Babolsar 47416-95447, Iran. hadjmr@umz.ac.ir
Journal Details
This article was published in the following journal.
Name: Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Pages: 44-9
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22172600
- DOI: http://dx.doi.org/10.1016/j.jpba.2011.11.019
Medical and Biotech [MESH] Definitions
Solid Phase Microextraction
A solventless sample preparation method, invented in 1989, that uses a fused silica fiber which is coated with a stationary phase. It is used for sample cleanup before using other analytical methods.
Chromatography, Gas
Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix.
Gels
Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.
Solid Phase Extraction
An extraction method that separates analytes using a solid phase and a liquid phase. It is used for preparative sample cleanup before analysis by CHROMATOGRAPHY and other analytical methods.
Interphase
The interval between two successive CELL DIVISIONS during which the CHROMOSOMES are not individually distinguishable. It is composed of the G phases (G1 PHASE; G0 PHASE; G2 PHASE) and S PHASE (when DNA replication occurs).
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