Red blood cell storage and cell morphology.
Summary of "Red blood cell storage and cell morphology."
Aim: In this study, we performed weekly assessment of morphology-related parameters through monitoring of CPD-SAGM leuco-filtered erythrocyte concentrates from blood withdrawal until the 42nd day of storage. Background: Liquid storage of red blood cells (RBCs) delivers a blood-derived therapeutic, which is safe, available, effective and affordable for most patients who need transfusion therapy in developed countries. However, a growing body of accumulating controversial evidences, from either biochemical or retrospective clinical studies, prompted safety concerns about longer stored RBCs. Methods: Statistical image analysis through scanning electron microscope was coupled to osmotic fragility and erythrocyte sedimentation rate. Results: We could observe that by day 21 more than 50% of RBCs displayed non-discocyte phenotypes. This observation was related to an increase in osmotic fragility, which was totally overlapped in day 0 controls and day 7 RBCs while only slightly augmented in day 14 samples. Cation dysregulation (pH internal/external alteration and potassium) might both reflect and trigger a negative feedback loop with metabolic fluxes and membrane cation pumps. Conclusion: Morphology parameters suggest that significant alterations to RBC morphology over storage duration occur soon after the 14th day of storage, as to become significant enough within the 21st day.
Department of Ecological and Biological Sciences, Tuscia University, Largo dell'Universitá snc, Viterbo Celio Military Hospital, Sanitá Militare Dipartimento di Medicina Trasfusionale del Policlinico Militare, Rome, Italy.
This article was published in the following journal.
Name: Transfusion medicine (Oxford, England)
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22394111
- DOI: http://dx.doi.org/10.1111/j.1365-3148.2012.01139.x
To identify the impact of storage time and amount of transfused red blood cell units on renal function.
Thyroid hormones have a crucial role in metabolism and proliferation of blood cells. Thyroid dysfunction induces different effects on blood cells such as anemia, erythrocytosis leukopenia, thrombocyto...
The blood storage lesion involves morphological and biochemical changes of red blood cells (RBCs) that occur during storage. These include conversion of the biconcave disc morphology to a spherical on...
Renal involvement is frequent in hematologic malignancies especially Hodgkin's lymphoma. Renal complications in children with malignancies primarily arise from tumour lysis syndrome, malignant infiltr...
The RECESS study will compare the effects of transfusing red blood cell units stored = 21 days, in patients who are undergoing complex cardiac surgery and are likely to need a red blood cell tra...
The purpose of this study is to determine the safety and engraftment of donor hematopoietic cells using this conditioning regimen in patients undergoing a hematopoietic (blood forming) cel...
This study seeks to investigate the factors that may account for the differences in the way a stem cell recipient's immune system works after a transplant. It will focus on cytokine genes...
This study will determine the best ways to collect, process and store umbilical cord blood from babies with sickle cell disease, sickle cell trait and unaffected babies. Sickle cell disea...
This is a clinical trial of bone marrow transplantation for patients with the diagnosis of a genetic disease of blood cells that do not have an HLA-matched sibling donor. Genetic diseases...
Medical and Biotech [MESH] Definitions
A group of malignant lymphomas thought to derive from peripheral T-lymphocytes in lymph nodes and other nonlymphoid sites. They include a broad spectrum of lymphocyte morphology, but in all instances express T-cell markers admixed with epithelioid histiocytes, plasma cells, and eosinophils. Although markedly similar to large-cell immunoblastic lymphoma (LYMPHOMA, LARGE-CELL, IMMUNOBLASTIC), this group's unique features warrant separate treatment.
The mechanisms by which a cell becomes internalized in another. The host cell may engulf another as do PHAGOCYTIC CELLS, or the host cell may be invaded by another cell (ENTOSIS), or internalization processes may involve the cooperation of both the host cell and the cell being internalized. Viable cells may remain in non-phagocytic cells (EMPERIPOLESIS), undergo cell division, pass through and then out of the host cell (TRANSCELLULAR CELL MIGRATION), or trigger APOPTOSIS of the invaded cell.
The decrease in the cell's ability to proliferate with the passing of time. Each cell is programmed for a certain number of cell divisions and at the end of that time proliferation halts. The cell enters a quiescent state after which it experiences CELL DEATH via the process of APOPTOSIS.
A group of carcinomas which share a characteristic morphology, often being composed of clusters and trabecular sheets of round "blue cells", granular chromatin, and an attenuated rim of poorly demarcated cytoplasm. Neuroendocrine tumors include carcinoids, small ("oat") cell carcinomas, medullary carcinoma of the thyroid, Merkel cell tumor, cutaneous neuroendocrine carcinoma, pancreatic islet cell tumors, and pheochromocytoma. Neurosecretory granules are found within the tumor cells. (Segen, Dictionary of Modern Medicine, 1992)
A genus of gram-negative, chemoorganotrophic bacteria in the family Flexibacteraceae. In some species there is a cyclic change in cell morphology.