Covalent immobilization of Kluyveromyces fragilis β-galactosidase on magnetic nanosized epoxy support for synthesis of galacto-oligosaccharide.
Summary of "Covalent immobilization of Kluyveromyces fragilis β-galactosidase on magnetic nanosized epoxy support for synthesis of galacto-oligosaccharide."
The novel magnetic nanobeads with epoxy groups on the surface were prepared from glycidyl methacrylate (GMA), ethylene glycol dimethacrylate (EGDMA) and hydroxyethyl methacrylate (HEMA) via emulsifier-free emulsion polymerisation, and they were characterized by scanning electron microscopy and vibrating sample magnetometer. The magnetic poly(GMA-EDGMA-HEMA) nanobeads were used as support for covalent immobilization of Kluyveromyces fragilis β-galactosidase, the maximum amount enzyme attached onto the support was 145.6 mg/g with activity recovery of 72.6%. The loading capacity of this novel support for K. fragilis β-galactosidase was improved 2.6-folds compared with Eupergit(®) C (commercial epoxy support). The immobilized K. fragilis β-galactosidase exhibited high catalytic activity for the reaction of galacto-oligosaccharide (GOS) synthesis, and a total of 2,240 g GOS were produced per gram of immobilized enzyme during consecutive batch reaction of 10 times. The immobilized biocatalyst retained 81.5% of its original activity after 10 reaction cycles.
Affiliation
Agricultural Information Institute of Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Journal Details
This article was published in the following journal.
Name: Bioprocess and biosystems engineering
ISSN: 1615-7605
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22398930
- DOI: http://dx.doi.org/10.1007/s00449-012-0716-2
Medical and Biotech [MESH] Definitions
Epoxy Resins
Organic compounds containing an epoxide group and characterized by strength and thermosetting properties. Epoxy resins are often used as dental materials.
Beta-galactosidase
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
Kluyveromyces
An ascomycetous yeast of the fungal family Saccharomycetaceae, order SACCHAROMYCETALES.
Lincomycin
An antibiotic produced by Streptomyces lincolnensis var. lincolnensis. It has been used in the treatment of staphylococcal, streptococcal, and Bacteroides fragilis infections.
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