Rational aspect ratio and suitable antibody coverage of gold nanorod for ultra-sensitive detection of a cancer biomarker.

14:16 EDT 25th October 2014 | BioPortfolio

Summary of "Rational aspect ratio and suitable antibody coverage of gold nanorod for ultra-sensitive detection of a cancer biomarker."

We report a simple, ultra-sensitive, and straightforward method for non-labeling detection of a cancer biomarker, using Rayleigh light scattering spectroscopy of the individual nanosensor based on antibody-antigen recognition and localized surface plasmon resonance (LSPR) λ(max) shifts. By experimentally measuring the refractive index sensitivity of Au nanorods, the Au nanorod with an aspect ratio of ∼3.5 was proven optimal for the LSPR sensing. To reduce the steric hindrance effect as well as to immobilize a large amount of ligand on the nanoparticle surface, various mixtures containing different molar ratios of HS(CH(2))(11)(OCH(2)CH(2))(6)OCH(2)COOH and HS(CH(2))(11)(OCH(2)CH(2))(3)OH were applied to form different self-assembled monolayer surfaces. The results showed that the best molar ratio for antibody conjugation was 1 : 10. When using individual Au nanorod sensors for the detection of prostate specific antigen (PSA), the lowest concentration recorded was ∼1 aM (∼6 × 10(5) molecules), corresponding to LSPR λ(max) shifts of ∼4.2 nm. These results indicate that sensor miniaturization down to the nanoscale level, the reduction of steric hindrance, and optimization of size, shape, and aspect ratio of nanorods have led to a significant improvement in the detection limit of sensors.

Affiliation

School of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, Korea.

Journal Details

This article was published in the following journal.

Name: Lab on a chip
ISSN: 1473-0189
Pages: 1102-9

Links

PubMed Articles [12532 Associated PubMed Articles listed on BioPortfolio]

Polyethylenimine-Assisted Growth of High-Aspect-Ratio Nitrogen-Doped ZnO (NZO) Nanorod Arrays and Their Effect on Performance of Dye-Sensitized Solar Cells.

The realization of arrays of high-aspect-ratio nitrogen-doped ZnO (NZO) nanorod is critical to the development of high-quality nanostructure-based optoelectronic and electronic devices. In this study,...

In situ observation of structural transformation of gold nanorods under pulsed laser irradiation in an HVEM.

A pulsed laser light illumination system was attached to a high-voltage electron microscope (HVEM) for in situ observation of light-induced behaviors of nano objects. The wavelength λ of emitted lase...

Impact of filling ratio on subwavelength optical imaging using metallic nanolens of different geometries.

A nanolens based on a metallic nanorod has been considered as a prospective candidate for transporting subwavelength information. Such a lens is tuned to a particular frequency by tailoring the length...

High Aspect Ratio Wrinkles via Substrate Prestretch.

A non-fractured, high aspect ratio wrinkled surface is successfully fabricated. Building upon recently developed models of the localization transition and the current knowledge of surface failures, th...

Tailoring nanorod alignment in a polymer matrix by elongational flow under confinement: simulation, experiments, and surface enhanced Raman scattering application.

Mesoscale simulation, electrospinning and Raman scattering experiments have been carried out to demonstrate that examination and control of nanorod configuration in a polymer matrix under elongational...

Clinical Trials [1714 Associated Clinical Trials listed on BioPortfolio]

Optical Coherence Tomography to Evaluate Paclitaxel-Eluting Balloons and Everolimus-Eluting Coronary Stents

Background: Safety concerns regarding use of drug eluting stent systems (DES) are related mostly to late stent thrombosis, which is facilitated by incomplete stent endothelial coverage. S...

A Study to Describe Pediatric Influenza Vaccine Coverage

The purpose of this study is to describe influenza vaccine coverage in children less than 18 years of age and to examine correlations between in-office influenza vaccine coverage various ...

Comparison of Neointimal Coverage Between Zotarolimus Eluting Stent and Everolimus Eluting Stent

Stent thrombosis is an important issue in drug eluting stents. Incomplete endothelial coverage and neointimal coverage over strut after drug eluting stent (DES) implantation could be a pos...

Stool Composition and Stool Characteristics in Healthy Term Infants Fed Human Milk or Infant Formulas

The primary efficacy objective was to determine and compare stool composition (stool soap and non-soap fatty acids, total fatty acids, minerals, and other stool constituents) among the fee...

Gold Against Pains From Osteoarthrosis in the Knee

Goldimplantation around arthritic joints is a very popular treatment - but the effect has never been controlled.

Medical and Biotech [MESH] Definitions

Precipitin tests which occur over a narrow range of antigen-antibody ratio, due chiefly to peculiarities of the antibody (precipitin). (From Stedman, 26th ed)

The ratio of two odds. The exposure-odds ratio for case control data is the ratio of the odds in favor of exposure among cases to the odds in favor of exposure among noncases. The disease-odds ratio for a cohort or cross section is the ratio of the odds in favor of disease among the exposed to the odds in favor of disease among the unexposed. The prevalence-odds ratio refers to an odds ratio derived cross-sectionally from studies of prevalent cases.

Unstable isotopes of gold that decay or disintegrate emitting radiation. Au 185-196, 198-201, and 203 are radioactive gold isotopes.

Stable gold atoms that have the same atomic number as the element gold, but differ in atomic weight. Au-197 is a stable isotope.

A suspension of metallic gold particles.

Search BioPortfolio:
Loading
Advertisement

Relevant Topics

Cancer Disease
Latest News Clinical Trials Research Drugs Reports Corporate
Cancer is not just one disease but many diseases. There are more than 100 different types of cancer. Most cancers are named for the organ or type of cell in which they start - for example, cancer that begins in the colon is called colon cancer; cancer th...

Cancer
Latest News Clinical Trials Research Drugs Reports Corporate
Cancer is a condition where cells in a specific part of the body grow and reproduce uncontrollably. The cancerous cells can invade and destroy surrounding healthy tissue, including organs.  Cancer sometimes begins in one part of the body before spre...

Antibodies
Latest News Clinical Trials Research Drugs Reports Corporate
An antibody is a protein produced by the body's immune system when it detects harmful substances, called antigens. Examples of antigens include microorganisms (such as bacteria, fungi, parasites, and viruses) and chemicals. Antibodies may be produc...

Advertisement

Searches Linking to this Article