Interaction Between 2',4-Dihydroxychalcone and the N, F, E Conformers of Bovine Serum Albumin: Influence of Temperature and Ionic Strength.
Summary of "Interaction Between 2',4-Dihydroxychalcone and the N, F, E Conformers of Bovine Serum Albumin: Influence of Temperature and Ionic Strength."
UV-Vis spectroscopy was used to study the interaction between the 2',4- dihydroxychalcone, flavonoid which is known to have anti-tumor activity in vitro, and others biological properties, and the N, F and E conformers of bovine serum albumin at different ionic strengths and temperatures. The Klotz model was found to be adequate to determine the constants and number of binding sites. The reaction was found to be exothermic and spontaneous. The number of binding sites decreases and the reaction is more exergonic along with the increase in ionic strength and the conformational change of N to E. The reactions were necessarily hydrophobic and followed by a process of ionic character.
Affiliation
Area of Chemistry and Physics, Faculty of Chemistry, Biochemistry and Pharmacy, Universidad Nacional de San Luis, San Luis, Argentina, rcurvale@unsl.edu.ar.
Journal Details
This article was published in the following journal.
Name: The protein journal
ISSN: 1875-8355
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22450828
- DOI: http://dx.doi.org/10.1007/s10930-012-9404-y
Medical and Biotech [MESH] Definitions
Serum Albumin, Bovine
Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)
Hypoalbuminemia
A condition in which albumin level in blood (SERUM ALBUMIN) is below the normal range. Hypoalbuminemia may be due to decreased hepatic albumin synthesis, increased albumin catabolism, altered albumin distribution, or albumin loss through the urine (ALBUMINURIA).
Serum Globulins
All blood proteins except albumin ( = SERUM ALBUMIN, which is not a globulin) and FIBRINOGEN (which is not in the serum). The serum globulins are subdivided into ALPHA-GLOBULINS; BETA-GLOBULINS; and GAMMA-GLOBULINS on the basis of their electrophoretic mobilities. (From Dorland, 28th ed)
Serum Albumin, Radio-iodinated
Normal human serum albumin mildly iodinated with radioactive iodine (131-I) which has a half-life of 8 days, and emits beta and gamma rays. It is used as a diagnostic aid in blood volume determination. (from Merck Index, 11th ed)
Radioimmunoassay
Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.
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