Successful Production of Offspring Using Cryopreserved Sperm via Nonsurgical Artificial Insemination in Rats.
Summary of "Successful Production of Offspring Using Cryopreserved Sperm via Nonsurgical Artificial Insemination in Rats."
In rats, artificial insemination (AI) is surgically performed as a general tool to obtain offspring using cryopreserved spermatozoa. Nonsurgical AI is a more desirable technology because it does not require any surgical procedures. However, there has never been a successful nonsurgical AI since frozen-thawed rat spermatozoa show low motility. We show here for the first time successful nonsurgical AI in rats using oxytocin treatment. Intraperitoneal injection of oxytocin (1/800 I.U.) immediately before nonsurgical AI significantly increased the number of sperm collected from the oviducts compared with that without oxytocin treatment. Therefore, to obtain pups, oxytocin was intraperitoneally injected into females mated with vasectomized males, and the rats were then used for nonsurgical AI. Seven of the 12 oxytocin-treated rats became pregnant after nonsurgical AI, and 37 pups were obtained. Only one rat (1/13) without oxytocin treatment was pregnant after nonsurgical AI, and only 1 pup was delivered. These results show success for the first time in obtaining offspring using frozen-thawed rat spermatozoa via nonsurgical AI. Our results also suggest the possibility that oxytocin treatment is effective for improvement of nonsurgical AI even in other species.
Affiliation
Laboratory of Animal Reproduction, Graduate School of Veterinary Science, Azabu University, Sagamihara 252-5201, Japan.
Journal Details
This article was published in the following journal.
Name: The Journal of reproduction and development
ISSN: 1348-4400
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22452831
- DOI: http://dx.doi.org/
Medical and Biotech [MESH] Definitions
Semen Analysis
The quality of SEMEN, an indicator of male fertility, can be determined by semen volume, pH, sperm concentration (SPERM COUNT), total sperm number, sperm viability, sperm vigor (SPERM MOTILITY), normal sperm morphology, ACROSOME integrity, and the concentration of WHITE BLOOD CELLS.
Sex Determination (genetics)
The mechanism in a given species, by which the SEX of an individual is fixed. In many species, sex is established at FERTILIZATION by the SPERM that fertilizes the EGG. In human, the Y CHROMOSOME bearing sperm determine production of the male ZYGOTE.
Sperm Injections, Intracytoplasmic
An assisted fertilization technique consisting of the microinjection of a single viable sperm into an extracted ovum. It is used principally to overcome low sperm count, low sperm motility, inability of sperm to penetrate the egg, or other conditions related to male infertility (INFERTILITY, MALE).
Sperm-ovum Interactions
Interactive processes between the oocyte (OVUM) and the sperm (SPERMATOZOA) including sperm adhesion, ACROSOME REACTION, sperm penetration of the ZONA PELLUCIDA, and events leading to FERTILIZATION.
Oligospermia
A condition of suboptimal concentration of SPERMATOZOA in the ejaculated SEMEN to ensure successful FERTILIZATION of an OVUM. In humans, oligospermia is defined as a sperm count below 20 million per milliliter semen.
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