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ISOLATION AND CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM HUMAN AMNIOTIC MEMBRANE.

06:00 EDT 3rd August 2010 | BioPortfolio

Summary of "ISOLATION AND CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM HUMAN AMNIOTIC MEMBRANE."

Introduction: The human amniotic membrane (HAM), a highly abundant and readily available tissue that may be useful for regenerative medicine and cell therapy. Aim: To compare two previously published protocols for the isolation of human amnion mesenchymal stromal cells (hAMSCs), including their phenotypic characterization and in vitro potential for differentiation toward osteogenic, adipogenic and chondrogenic mesodermal lineages. Materials and Methods: Human placentas were obtained from selected caesarean-sectioned births. Two different protocols (Alviano et al. [1] and Soncini et al. [2]) for the isolation of hAMSCs were performed. After monolayer expansion of adherent cells from both protocols, the cells were characterized by flow cytometry and for multipotentiality, as assessed by their capability to differentiate toward adipocyte-, osteoblast- and chondocyte-like cells. Results: Both protocols yielded hAMSCs that showed plastic adherence, fibroblast-like growth and well-defined human MSC markers. The cell yield and mesodermal differentiation capability of hAMSCs were higher in cells isolated using the Soncini protocol. Conclusions: Our data demonstrated the successful isolation of hAMSCs from full-term placentas using two published protocols. Differences between the two protocols in cell yield and in vitro differentiation potential were shown.

Affiliation

INIBIC-Universidade da Coruña, Departamento de Medicina, A Coruña, A Coruña, Spain; silvia.diaz@sergas.es.

Journal Details

This article was published in the following journal.

Name: Tissue engineering. Part C, Methods
ISSN: 1937-3392
Pages:

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