Piperine, a component of black pepper, inhibits adipogenesis by antagonizing PPARγ activity in 3T3-L1 cells.
Summary of "Piperine, a component of black pepper, inhibits adipogenesis by antagonizing PPARγ activity in 3T3-L1 cells."
Black pepper (Piper nigrum Linne) has been world-widely used as spice and herbal medicine because of its anti-oxidant, anti-inflammation, and anti-tumor activities. In this study, we investigated the anti-adipogenic activity of black pepper extract and its constituent piperine in 3T3-L1 preadipocytes, and the underlying molecular mechanisms. Both black pepper extract and piperine, without affecting cytotoxicity, strongly inhibited the adipocyte differentiation of 3T3-L1 cells as evidenced by Oil Red O staining and levels of accumulated triglyceride. The mRNA expression of the master adipogenic transcription factors, PPARγ, SREBP-1c, and C/EBPα, was markedly decreased, especially upon treating piperine. Intriguingly, mRNA levels of PPARγ target genes such as adipsin, aP2, and LPL were also down-regulated. Moreover, co-transfection assays using luciferase reporter genes indicated that pipierine significantly represses the rosiglitazone-induced PPARγ transcriptional activity. Finally, GST-pull down assays demonstrated that piperine disrupts the rosiglitazone-dependent interaction between PPARγ and coactivator CBP. Genome-wide analysis using microarray further supports the role of piperine act as an antagonist of PPARγ. Overall, these results suggest that piperine, a major component of black pepper, attenuates fat cell differentiation by down-regulating PPARγ activity as well as suppressing PPARγ expression, thus leading to potential treatment for obesity-related diseases.
Affiliation
Journal Details
This article was published in the following journal.
Name: Journal of agricultural and food chemistry
ISSN: 1520-5118
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22463744
- DOI: http://dx.doi.org/10.1021/jf204514a
Medical and Biotech [MESH] Definitions
Black Pepper
A common spice from fruit of PIPER NIGRUM. Black pepper is picked unripe and heaped for a few days to ferment. White Pepper is the ripe fruit dehulled by maceration in water. Piperine is a key component used medicinally to increase gastrointestinal assimilation of other supplements and drugs.
Piper Nigrum
A plant species in the PIPERACEAE plant family. It is a common spice on foods and is used medicinally to increase gastrointestinal assimilation of other supplements and drugs. Piperine is a key component. Black pepper is picked unripe and heaped for a few days to ferment. White Pepper is the ripe fruit dehulled by maceration in water.
Piperaceae
A family of flowering plants in the order Piperales best known for the black pepper widely used in SPICES, and for KAVA and Betel used for neuroactive properties.
Ppar-beta
One of the PPAR nuclear transcription factors.
Serum Amyloid P-component
Amyloid P component is a small, non-fibrillar glycoprotein found in normal serum and in all amyloid deposits. It has a pentagonal (pentaxin) structure. It is an acute phase protein, modulates immunologic responses, inhibits ELASTASE, and has been suggested as an indicator of LIVER DISEASE.
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