A "signal-on" electrochemical aptasensor for simultaneous detection of two tumor markers.
Summary of "A "signal-on" electrochemical aptasensor for simultaneous detection of two tumor markers."
In this paper, we report a "signal-on" electrochemical aptasensor for simultaneous determination of two tumor markers MUC1 and VEGF(165), by using a ferrocene-labeled aptamer-complementary DNA (cDNA) as probe. Since the cDNA immobilized on an electrode surface can hybridize with both MUC1 aptamer and VEGF(165) aptamer to form a long double strand with ferrocene far away from the electrode surface, the probe cannot give electrochemical signal. Nevertheless, the presence of the two tumor markers will inhibit the hybridization of cDNA with the aptamers, thus the distance between ferrocene and the electrode is changed, and a "signal-on" electrochemical method to detect two tumor markers is developed. Experimental results show that the electrochemical signal increases with the addition of either tumor markers, but the biggest electrochemical signal can only be obtained when both tumor markers are present. Therefore, the proposed electrochemical aptasensor can not only detect the two markers but also distinguish their co-existence. It may also display high selectivity and sensitivity towards the detection of the tumor markers, so it might have potential clinical application in the future.
Affiliation
Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, PR China.
Journal Details
This article was published in the following journal.
Name: Biosensors & bioelectronics
ISSN: 1873-4235
Pages: 249-52
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22386488
- DOI: http://dx.doi.org/10.1016/j.bios.2012.02.016
Medical and Biotech [MESH] Definitions
Neoplasm Proteins
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (TUMOR MARKERS, BIOLOGICAL) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Multiphasic Screening
The simultaneous use of multiple laboratory procedures for the detection of various diseases. These are usually performed on groups of people.
Signal Detection, Psychological
Psychophysical technique that permits the estimation of the bias of the observer as well as detectability of the signal (i.e., stimulus) in any sensory modality. (From APA, Thesaurus of Psychological Index Terms, 8th ed.)
Antigens, Tumor-associated, Carbohydrate
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
Branched Dna Signal Amplification Assay
A molecular probe technique that utilizes branched DNA (bDNA) as a means to amplify the hybridization signal. One end of the bDNA molecule is designed to bind a specific target, while the other end of the bDNA molecule contains many branches of DNA that are designed to bind a probe used for signal detection.
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