PCR detection of the crayfish plague pathogen in narrow-clawed crayfish inhabiting Lake EÄŸirdir in Turkey.
Summary of "PCR detection of the crayfish plague pathogen in narrow-clawed crayfish inhabiting Lake EÄŸirdir in Turkey."
Many populations of the narrow-clawed crayfish Astacus leptodactylus in Turkey, including those inhabiting Lake EÄŸirdir, declined drastically in the mid-1980s due to introduction of crayfish plague Aphanomyces astaci. However, unlike many other localities, there has been some recovery in the A. leptodactylus population inhabiting this lake even though crayfish plague has been suspected to have persisted since then. In support of this, DNA from 5 of 34 healthy-looking crayfish sampled recently from the lake tested positive by both conventional and real-time PCR using species-specific primers targeting the rDNA internal transcribed spacer region, and product sequence analysis confirmed the identification of A. astaci. This complies with other recent reports of coexistence of native European crayfish with this pathogen, and further research is now needed to identify the key mechanisms allowing it.
Affiliation
Department of Ecology, Faculty of Science, Charles University in Prague, 128 44 Prague 2, Czech Republic.
Journal Details
This article was published in the following journal.
Name: Diseases of aquatic organisms
ISSN: 0177-5103
Pages: 255-9
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22535876
- DOI: http://dx.doi.org/10.3354/dao02445
Medical and Biotech [MESH] Definitions
Astacoidea
A superfamily of various freshwater CRUSTACEA, in the infraorder Astacidea, comprising the crayfish. Common genera include Astacus and Procambarus. Crayfish resemble lobsters, but are usually much smaller.
Decapoda (crustacea)
The largest order of CRUSTACEA, comprising over 10,000 species. They are characterized by three pairs of thoracic appendages modified as maxillipeds, and five pairs of thoracic legs. The order includes the familiar shrimps, crayfish (ASTACOIDEA), true crabs (BRACHYURA), and lobsters (NEPHROPIDAE and PALINURIDAE), among others.
Plague Vaccine
A suspension of killed Yersinia pestis used for immunizing people in enzootic plague areas.
Plague
An acute infectious disease caused by YERSINIA PESTIS that affects humans, wild rodents, and their ectoparasites. This condition persists due to its firm entrenchment in sylvatic rodent-flea ecosystems throughout the world. Bubonic plague is the most common form.
Substance Abuse Detection
Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.
PubMed Articles
SUMMARY The crayfish plague pathogen, Aphanomyces astaci, is one of the most serious threats to indigenous European crayfish species. The North American invasive spiny-cheek crayfish, Orconectes limos...
Dominance hierarchy-dependent behavioural plasticity of crayfish avoidance reactions.
Crayfish showed avoidance reactions when mechanical stimulation was applied to their tailfan. The response pattern of the avoidance reaction was dependent on crayfish size. Small crayfish showed an es...
Laboratory experiments were performed to examine the toxic effects of fluoride (F(-)) on the survival and behavior of white-clawed crayfish (Austropotamobius pallipes). Body fluoride contents (bioaccu...
Aphanomyces astaci sporulation is crucial for the spreading potential of this disease agent. For the first time, we are reporting timing and quantity of A. astaci spores released from noble crayfish (...
On June 24, 2010, the Spokane (Washington) Regional Health District (SRHD) was notified of two hospitalized patients under intensive care with severe dehydration whose stool specimens yielded Vibrio m...
Clinical Trials
The overall objective of this study is to assess the clinical value of the SeptiFast Test as an adjunct to traditional microbiological, clinical, and other laboratory assessments in early...
Treatment and Diagnosis of Plague
This clinical trial will compare the effectiveness of streptomycin, which historically is the standard drug for treatment of plague, with gentamicin. The hypothesis is that gentamicin is...
One hundred and five subjects will be recruited into three groups. Each subject will receive two doses of recombinant plague vaccine at one of three dose levels (rF1 and rV recombinant an...
The Use of Narrow Band Imaging in Screening Colonoscopy
Narrow band imaging (NBI) is an imaging technique that allows a better definition of capillary pattern and improves the contrast between adenomas and the surrounding mucosa. Conflicting da...
Objective: To determine if platelets treated for pathogen inactivation and stored for 6 to 7 days are safe and effective compared to platelets collected by the same method, stored for the...
