PCR detection of the crayfish plague pathogen in narrow-clawed crayfish inhabiting Lake E─čirdir in Turkey.
Summary of "PCR detection of the crayfish plague pathogen in narrow-clawed crayfish inhabiting Lake E─čirdir in Turkey."
Many populations of the narrow-clawed crayfish Astacus leptodactylus in Turkey, including those inhabiting Lake E─čirdir, declined drastically in the mid-1980s due to introduction of crayfish plague Aphanomyces astaci. However, unlike many other localities, there has been some recovery in the A. leptodactylus population inhabiting this lake even though crayfish plague has been suspected to have persisted since then. In support of this, DNA from 5 of 34 healthy-looking crayfish sampled recently from the lake tested positive by both conventional and real-time PCR using species-specific primers targeting the rDNA internal transcribed spacer region, and product sequence analysis confirmed the identification of A. astaci. This complies with other recent reports of coexistence of native European crayfish with this pathogen, and further research is now needed to identify the key mechanisms allowing it.
Department of Ecology, Faculty of Science, Charles University in Prague, 128 44 Prague 2, Czech Republic.
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Name: Diseases of aquatic organisms
Medical and Biotech [MESH] Definitions
A superfamily of various freshwater CRUSTACEA, in the infraorder Astacidea, comprising the crayfish. Common genera include Astacus and Procambarus. Crayfish resemble lobsters, but are usually much smaller.
The largest order of CRUSTACEA, comprising over 10,000 species. They are characterized by three pairs of thoracic appendages modified as maxillipeds, and five pairs of thoracic legs. The order includes the familiar shrimps, crayfish (ASTACOIDEA), true crabs (BRACHYURA), and lobsters (NEPHROPIDAE and PALINURIDAE), among others.
A suspension of killed Yersinia pestis used for immunizing people in enzootic plague areas.
An acute infectious disease caused by YERSINIA PESTIS that affects humans, wild rodents, and their ectoparasites. This condition persists due to its firm entrenchment in sylvatic rodent-flea ecosystems throughout the world. Bubonic plague is the most common form.
Substance Abuse Detection
Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.
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