A novel method for infecting Drosophila adult flies with insect pathogenic nematodes.
Summary of "A novel method for infecting Drosophila adult flies with insect pathogenic nematodes."
Drosophila has been established as an excellent genetic and genomic model to investigate host-pathogen interactions and innate immune defense mechanisms. To date, most information on the Drosophila immune response derives from studies that involve bacterial, fungal or viral pathogens. However, immune reactions to insect parasitic nematodes are still not well characterized. The nematodes Heterorhabditis bacteriophora live in symbiosis with the entomopathogenic bacteria Photorhabdus luminescens, and they are able to invade and kill insects. Interestingly, Heterorhabditis nematodes are viable in the absence of Photorhabdus. Techniques for infecting Drosophila larvae with these nematodes have been previously reported. Here, we have developed a method for infecting Drosophila adult flies with Heterorhabditis nematodes carrying (symbiotic worms) or lacking (axenic worms) their associated bacteria. The protocol we present can be readily adapted for studying parasitic strategies of other insect nematodes using Drosophila as the host infection model.
Affiliation
Insect Infection and Immunity Lab; Department of Biological Sciences; The George Washington University; Washington, DC USA.
Journal Details
This article was published in the following journal.
Name: Virulence
ISSN: 2150-5608
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22546901
- DOI: http://dx.doi.org/
Medical and Biotech [MESH] Definitions
Drosophila Proteins
Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.
Diflubenzuron
An insect growth regulator which interferes with the formation of the insect cuticle. It is effective in the control of mosquitoes and flies.
Entomobirnavirus
A genus of RNA viruses of the family BIRNAVIRIDAE infecting fruitflies. Transmission is horizontal and there are no known vectors. Drosophila melanogaster is the natural host and the type species is Drosophila X virus.
Drosophila
A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.
Son Of Sevenless Protein, Drosophila
A guanine nucleotide exchange factor from DROSOPHILA. Sevenless refers to genetic mutations in DROSOPHILA that cause loss of the R7 photoreceptor which is required to see UV light.
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