Expression of selected genes in pre-term premature rupture of fetal membranes.
Summary of "Expression of selected genes in pre-term premature rupture of fetal membranes."
Objective. To analyze the expression of 15 genes encoding receptors and enzymes associated with the molecular mechanism of the tocolytic drugs atosiban (oxytocin receptor antagonist), nifedipine (calcium channel blocker), and celecoxib (selective cyclooxygenase-2 inhibitor) in pre-term labor patients with premature rupture of fetal membranes in relation to symptoms of intrauterine infection and pre-term labor risk factors. Design. Experimental molecular study. Setting. Tertiary obstetric care center. Sample. Myometrial samples were obtained during cesarean sections from 35 patients who delivered pre-term with unverified symptoms of intrauterine infection, 35 patients who delivered pre-term without symptoms of intrauterine infection, and 90 women who delivered at term. Methods. The Micro Fluidic Profiling Card analytic system was used to evaluate mRNA expression of the genes of interest. Main outcome measures. The relative quantification values for mRNA expression. Results. The median oxytocin receptor and cyclooxygenase-2 mRNA expression in pre-term patients with clinical symptoms of intrauterine infection was significantly higher than in pre-term patients without symptoms. The median mRNA expression of β(1,) β(3,) and β(4) subunits of calcium channel type L and prostaglandin E(2) receptor was significantly higher in pre-term patients compared to term patients. Conclusions. mRNA expression of hormones, enzymes, and their receptors associated with tocolytic actions can differ under various clinical conditions. The expression of these genes are regulated at different levels and can be modified by inflammatory factors which affect their functions.
Affiliation
Department of Perinatology,and Department of Clinical Molecular Biology, Medical University of Białystok, Białystok, Poland.
Journal Details
This article was published in the following journal.
Name: Acta obstetricia et gynecologica Scandinavica
ISSN: 1600-0412
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22568870
- DOI: http://dx.doi.org/10.1111/j.1600-0412.2012.01445.x
Medical and Biotech [MESH] Definitions
Fetal Membranes, Premature Rupture
Spontaneous tearing of the membranes surrounding the FETUS any time before the onset of OBSTETRIC LABOR. Preterm PROM is membrane rupture before 37 weeks of GESTATION.
Obstetric Labor, Premature
Onset of OBSTETRIC LABOR before term (TERM BIRTH) but usually after the FETUS has become viable. In humans, it occurs sometime during the 29th through 38th week of PREGNANCY. TOCOLYSIS inhibits premature labor and can prevent the BIRTH of premature infants (INFANT, PREMATURE).
Epistasis, Genetic
A form of gene interaction whereby the expression of one gene interferes with or masks the expression of a different gene or genes. Genes whose expression interferes with or masks the effects of other genes are said to be epistatic to the effected genes. Genes whose expression is affected (blocked or masked) are hypostatic to the interfering genes.
Genes, Immediate-early
Genes that show rapid and transient expression in the absence of de novo protein synthesis. The term was originally used exclusively for viral genes where immediate-early referred to transcription immediately following virus integration into the host cell. It is also used to describe cellular genes which are expressed immediately after resting cells are stimulated by extracellular signals such as growth factors and neurotransmitters.
Vasa Previa
Pregnancy complication where fetal blood vessels, normally inside the umbilical cord, are left unprotected and cross FETAL MEMBRANES. It is associated with antepartum bleeding and FETAL DEATH and STILLBIRTH due to exsanguination.
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