Isolation Of New Pseudomonas Tolaasii Bacteriophages And Genomic Investigation Of The Lytic Phage Bf7.
Summary of "Isolation Of New Pseudomonas Tolaasii Bacteriophages And Genomic Investigation Of The Lytic Phage Bf7."
Sixteen lytic bacteriophages that infect Pseudomonas tolaasii LMG 2342(T) were isolated from smashed sporocarps of oyster mushroom (Pleurotus ostreatus) showing necrotic symptoms. Based on the host range investigation of the phages they have wide infection abilities against the genus Pseudomonas, mainly in the case of phages Bf3, Bf7, Bf10 and Bf15. Molecular investigations have revealed that they all have dsDNA genomes about 40 kbp in size. Identical restriction patterns resulting from restriction enzyme analysis suggest that the isolates probably belong to the same phage species. However, there was a difference between these phage isolates in their infecting abilities. Phage isolate Bf7 was investigated and characterized more deeply. Morphological characterization of Bf7 by Transmission Electron Microscopy (TEM) has shown that it has a short, non-contractile tail, an icosahedral phage head and the size is about 60 nm in diameter, suggesting that it belongs to the Podoviridae family. Complete genome sequence analysis of the Bf7 phage isolate revealed a 40058 bp genome, 58.4% G+C content, 46 open reading frames encoding different proteins showing homology to proteins of the bacteriophage Caulobacter crescentus φCd1 from the Podoviridae family. On the basis of these results and comparative genomic studies we classified the Bf7 phage to the subfamily of Autographivirinae, φKMV-like phages. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.
Affiliation
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Hungary.
Journal Details
This article was published in the following journal.
Name: FEMS microbiology letters
ISSN: 1574-6968
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22568623
- DOI: http://dx.doi.org/10.1111/j.1574-6968.2012.02592.x
Medical and Biotech [MESH] Definitions
Rna Phages
Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.
Pseudomonas Phages
Viruses whose host is Pseudomonas. A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6.
Bacteriolysis
Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.
Microviridae
A large family of lytic bacteriophages infecting enterobacteria; SPIROPLASMA; BDELLOVIBRIO; and CHLAMYDIA. It contains four genera: MICROVIRUS; Spiromicrovirus; Bdellomicrovirus; and Chlamydiamicrovirus.
Bacteriophage Pf1
A species of filamentous Pseudomonas phage in the genus INOVIRUS, family INOVIRIDAE.
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