Effects of 50 Hz Electromagnetic Fields on Human Epidermal Stem Cells Cultured on Collagen Sponge Scaffolds.
Summary of "Effects of 50 Hz Electromagnetic Fields on Human Epidermal Stem Cells Cultured on Collagen Sponge Scaffolds."
Abstract Purpose: This study is to investigate the effects of electromagnetic fields (EMF) on proliferation of epidermal stem cells (ESC), which could present a viable clinical option for skin tissue engineering. Materials and methods: The ESC obtained from human foreskin were grafted into type-I three- dimensional collagen sponge scaffolds, and then were exposed with EMF (frequency 50 Hz, intensity 5 mT) for 14 d (30 min per d). Meanwhile, the control group was set under the same conditions without EMF. The effects of EMF on growth and proliferation of ESC were analyzed with staining of hematoxylin and eosin (H&E) and 4',6-diamidino-2-phenylindole (DAPI) under microscope or scanning electron microscope. The data of DAPI staining for 2 d, 7 d, 10 d and 14 d were collected respectively to investigate the cells proliferation. Results: ESC cultured in collagen sponge scaffolds could be steady grown and EMF could promote ESC proliferation compared with control (P<0.05). Conclusions: EMF could significantly promote proliferation of ESC, which leads to a promising clinical option for skin tissue engineering.
Affiliation
Journal Details
This article was published in the following journal.
Name: International journal of radiation biology
ISSN: 1362-3095
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22568519
- DOI: http://dx.doi.org/10.3109/09553002.2012.692496
Medical and Biotech [MESH] Definitions
Epidermal Growth Factor
A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. EPIDERMAL GROWTH FACTOR exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and epithelial cells.
Cells, Cultured
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Multipotent Stem Cells
Specialized stem cells that are committed to give rise to cells that have a particular function; examples are MYOBLASTS; MYELOID PROGENITOR CELLS; and skin stem cells. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: http://www.nih.gov/news/stemcell/primer.htm)
Peripheral Blood Stem Cell Transplantation
Transplantation of stem cells collected from the peripheral blood. It is a less invasive alternative to direct marrow harvesting of hematopoietic stem cells. Enrichment of stem cells in peripheral blood can be achieved by inducing mobilization of stem cells from the BONE MARROW.
Hela Cells
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks (one of several pseudonyms). These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
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