Successive transformation of benzoapyrene by laccase of Trametes versicolor and pyrene-degrading Mycobacterium strains.
Summary of "Successive transformation of benzoapyrene by laccase of Trametes versicolor and pyrene-degrading Mycobacterium strains."
We previously hypothesized that polycyclic aromatic hydrocarbon (PAH)-degrading bacteria that produce laccase may enhance the degree of benzo[a]pyrene mineralization. However, whether the metabolites of benzo[a]pyrene oxidized by laccase can be further transformed by PAH degraders remains unknown. In this study, pyrene-degrading mycobacteria with diverse degradation properties were isolated and employed for investigating the subsequent transformation on the metabolites of benzo[a]pyrene oxidized by fungal laccase of Trametes versicolor. The results confirm the successive transformation of benzo[a]pyrene metabolites, 6-benzo[a]pyrenyl acetate, and quinones by Mycobacterium strains, and report the discovery of the involvement of a O-methylation mediated pathway in the process. In detail, the vast majority of metabolite 6-benzo[a]pyrenyl acetate was transformed into benzo[a]pyrene quinones or methoxybenzo[a]pyrene, via two distinct steps that were controlled by the catechol-O-methyltransferase mediated O-methylation, while quinones were reduced to dihydroxybenzo[a]pyrene and further transformed into dimethoxy derivatives.
Affiliation
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Beijing East Road, 71, Nanjing, 210008, People's Republic of China.
Journal Details
This article was published in the following journal.
Name: Applied microbiology and biotechnology
ISSN: 1432-0614
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22573273
- DOI: http://dx.doi.org/10.1007/s00253-012-4120-2
Medical and Biotech [MESH] Definitions
Trametes
A genus of fungi in the family Coriolaceae.
7,8-dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
7,8,8a,9a-Tetrahydrobenzo(10,11)chryseno (3,4-b)oxirene-7,8-diol. A benzopyrene derivative with carcinogenic and mutagenic activity.
Benzo(a)pyrene
A potent mutagen and carcinogen. It is a public health concern because of its possible effects on industrial workers, as an environmental pollutant, an as a component of tobacco smoke.
Methylosinus Trichosporium
A species of METHYLOSINUS which is capable of degrading trichloroethylene and other organic pollutants.
Transfection
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
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