Impedimetric immunosensor based on SWCNT-COOH modified gold microelectrodes for label-free detection of deep venous thrombosis biomarker.
Summary of "Impedimetric immunosensor based on SWCNT-COOH modified gold microelectrodes for label-free detection of deep venous thrombosis biomarker."
Measurement of D-dimer has subsequently become an essential element in the diagnostics of deep vein thrombosis and pulmonary embolism; in this context microelectrodes with an area of 9x10(-4)cm(2) were used to develop impedimetric immunosensor for detecting deep venous thrombosis biomarker (D-dimer). The biosensor is based on functionalized carbon nanotubes (SWCNT-COOH) where the antibody (anti-D-dimer) was immobilized by covalent binding. The electrical properties and the morphology of the biolayer were characterized by electrochemical impedance spectroscopy (EIS), cyclic voltammetry and atomic force spectroscopy (AFM). Impedimetric microimmunosensor allows to obtain sensitivity of 40.1kOmegamuM(-1) and detection limit of 0.1pg/mL (0.53fM) with linear range from 0.1pg/mL to 2mug/mL (0.53fM to 0.01muM). We demonstrate that using carbon nanotubes and microelectrodes, high sensitivity and dynamic range were obtained. The biosensor exhibited a short response time of 10min. Moreover, the studied immunosensor exhibits good reproducibility (R.S.D. 8.2%, n=4).
Affiliation
University of Lyon, Laboratory of Analytical Sciences, Claude Bernard University Lyon 1, 43 Boulevard 11 Novembre 1918, 69622 Villeurbanne Cedex, France; University of Monastir, Laboratory of Physical and Chemistry of Interfaces, Faculty of Sciences of Mo
Journal Details
This article was published in the following journal.
Name: Biosensors & bioelectronics
ISSN: 1873-4235
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/20678914
- DOI: http://dx.doi.org/10.1016/j.bios.2010.07.004
Medical and Biotech [MESH] Definitions
Metal Ceramic Alloys
The fusion of ceramics (porcelain) to an alloy of two or more metals for use in restorative and prosthodontic dentistry. Examples of metal alloys employed include cobalt-chromium, gold-palladium, gold-platinum-palladium, and nickel-based alloys.
Gold Radioisotopes
Unstable isotopes of gold that decay or disintegrate emitting radiation. Au 185-196, 198-201, and 203 are radioactive gold isotopes.
Gold Isotopes
Stable gold atoms that have the same atomic number as the element gold, but differ in atomic weight. Au-197 is a stable isotope.
Gold Colloid
A suspension of metallic gold particles.
Gold Compounds
Inorganic compounds that contain gold as an integral part of the molecule.
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