Development of ultra-high erucic acid oil in the industrial oil crop Crambe abyssinica.
Summary of "Development of ultra-high erucic acid oil in the industrial oil crop Crambe abyssinica."
Erucic acid (22β:β1) is a major feedstock for the oleochemical industry. In this study, a gene stacking strategy was employed to develop transgenic Crambe abyssinica lines with increased 22β:β1 levels. Through integration of the LdLPAAT, BnFAE1 and CaFAD2-RNAi genes into the crambe genome, confirmed by Southern blot and qRT-PCR, the average levels of 18β:β1, 18β:β2 and 18β:β3 were markedly decreased and that of 22β:β1 was increased from 60% in the wild type to 73% in the best transgenic line of T4 generation. In single seeds of the same line, the 22β:β1 level could reach 76.9%, an increase of 28.0% over the wild type. The trierucin amount was positively correlated to 22β:β1 in the transgenic lines. Unlike high erucic rapeseed, the wild-type crambe contains 22β:β1 in the seed phosphatidylcholine and in the sn-2 position of triacylglycerols (5% and 8%, respectively). The transgenic line with high 22β:β1 had decreased 22β:β1 level in phosphatidylcholine, and this was negatively correlated with the 22β:β1 level at the sn-2 position of TAG. The significances of this study include (i) achieving an unprecedented level of 22β:β1 in an oil crop; (ii) disclosing mechanisms in the channelling of a triacylglycerol-specific unusual fatty acid in oil seeds; (iii) indicating potential limiting factors involved in the erucic acid biosynthesis and paving the way for further increase of this acid and (iv) development of an added value genetically modified oil crop having no risk of gene flow into feed and food crops.
Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, Sweden Plant Research International, Wageningen, the Netherlands Institute of Biology I, Botany, RWTH Aachen University, Aachen, Germany.
This article was published in the following journal.
Name: Plant biotechnology journal
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22642539
- DOI: http://dx.doi.org/10.1111/j.1467-7652.2012.00709.x
Medical and Biotech [MESH] Definitions
A genus of SPONGES in the family Crambeidae characterized by desmoid spicules. The type species is Crambe crambe.
Worthless, damaged, defective, superfluous or effluent material from industrial operations. It represents an ecological problem and health hazard.
Spectrometry, Mass, Secondary Ion
A mass-spectrometric technique that is used for microscopic chemical analysis. A beam of primary ions with an energy of 5-20 kiloelectronvolts (keV) bombards a small spot on the surface of the sample under ultra-high vacuum conditions. Positive and negative secondary ions sputtered from the surface are analyzed in a mass spectrometer in regards to their mass-to-charge ratio. Digital imaging can be generated from the secondary ion beams and their intensity can be measured. Ionic images can be correlated with images from light or other microscopy providing useful tools in the study of molecular and drug actions.
cis-13-Docosenoic Acids. 22-Carbon monounsaturated, monocarboxylic acids.
A 21-amino acid peptide that circulates in the plasma, but its source is not known. Endothelin-3 has been found in high concentrations in the brain and may regulate important functions in neurons and astrocytes, such as proliferation and development. It also is found throughout the gastrointestinal tract and in the lung and kidney. (N Eng J Med 1995;333(6):356-63)
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