Non-invasive identification of proteoglycans and chondrocyte differentiation state by Raman microspectroscopy.
Summary of "Non-invasive identification of proteoglycans and chondrocyte differentiation state by Raman microspectroscopy."
Proteoglycans (PGs) are crucial extracellular matrix (ECM) components that are present in all tissues and organs. Pathological remodeling of these macromolecules can lead to severe diseases such as osteoarthritis or rheumatoid arthritis. To date, PG-associated ECM alterations are routinely diagnosed by invasive analytical methods. Here, we employed Raman microspectroscopy, a laser-based, marker-free and non-destructive technique that allows the generation of spectra with peaks originating from molecular vibrations within a sample, to identify specific Raman bands that can be assigned to PGs within human and porcine cartilage samples and chondrocytes. Based on the non-invasively acquired Raman spectra, we further revealed that a prolonged in vitro culture leads to phenotypic alterations of chondrocytes, resulting in a decreased PG synthesis rate and loss of lipid contents. Our results are the first to demonstrate the applicability of Raman microspectroscopy as an analytical and potential diagnostic tool for non-invasive cell and tissue state monitoring of cartilage in biomedical research. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
Affiliation
Fraunhofer Institute for Interfacial Engineering and Biotechnology (IGB), Dept. of Cell and Tissue Engineering, Nobelstr. 12, 70569 Stuttgart, Germany; University of Stuttgart, Institute for Interfacial Engineering (IGVT), Nobelstr. 12, 70569 Stuttgart, G
Journal Details
This article was published in the following journal.
Name: Journal of biophotonics
ISSN: 1864-0648
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22678997
- DOI: http://dx.doi.org/10.1002/jbio.201200064
Medical and Biotech [MESH] Definitions
Spectrum Analysis, Raman
Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.
Growth Differentiation Factor 3
A growth differentiation factor that may play a role in maintaining the undifferentiated state of PLURIPOTENT STEM CELLS by inhibiting the actions of BONE MORPHOGENETIC PROTEINS. Differentiation factor 3 is also considered a nodal signaling ligand that influences the genesis of left-right asymmetry.
Glypicans
A family of GLYCOSYLPHOSPHATIDYLINOSITOL-anchored, cell-surface heparan sulfate proteoglycans that may play a role in CELL GROWTH PROCESSES and CELL DIFFERENTIATION by modulating ligand-receptor interactions.
Chondroitin Sulfate Proteoglycans
Proteoglycans consisting of proteins linked to one or more CHONDROITIN SULFATE-containing oligosaccharide chains.
Receptor, Fibroblast Growth Factor, Type 3
A fibroblast growth factor receptor that regulates CHONDROCYTE growth and CELL DIFFERENTIATION. Mutations in the gene for fibroblast growth factor receptor 3 have been associated with ACHONDROPLASIA; THANATOPHORIC DYSPLASIA and NEOPLASTIC CELL TRANSFORMATION.
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