Cryopreservation of cell/scaffold tissue engineered constructs.
Summary of "Cryopreservation of cell/scaffold tissue engineered constructs."
The aim of this work was to study the effect of cryopreservation over the functionality of tissue engineered constructs, analysing the survival and viability of cells seeded, cultured and cryopreserved onto 3D scaffolds. Furthermore, it was also evaluated the effect of cryopreservation over the properties of the scaffold material itself since these are critical for the engineering of most tissues and in particular tissues such as i.e. bone. For this purpose, porous scaffolds, namely fiber meshes based on a starch and poly(caprolactone) blend (SPCL) were seeded with goat bone marrow stem cells (GBMSCs) and cryopreserved for 7 days. Discs of the same material seeded with GBMSCs were also used as controls. After this period, these samples were analyzed and compared to samples collected before the cryopreservation process. The obtained results demonstrate that it is possible to maintain cell viability and scaffolds properties upon cryopreservation of tissue engineered constructs based on starch scaffolds and goat bone marrow mesenchymal cells using standard cryopreservation methods. In addition, the outcomes of this study suggest that the greater porosity and interconnectivity of scaffolds favor the retention of cellular content and cellular viability during cryopreservation processes, when comparing to nonporous discs. These findings indicate that it might be possible to prepare off-the-shelf engineered tissue substitutes and preserve them in order to be immediately available upon patient's needs.
Affiliation
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Guimarães, Portugal; pedro.costa@dep.uminho.pt.
Journal Details
This article was published in the following journal.
Name: Tissue engineering. Part C, Methods
ISSN: 1937-3392
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22676448
- DOI: http://dx.doi.org/10.1089/ten.TEC.2011.0649
Medical and Biotech [MESH] Definitions
Cryopreservation
Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.
Dimethyl Sulfoxide
A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.
Tissue Scaffolds
Cell growth support structures composed of BIOCOMPATIBLE MATERIALS. They are specially designed solid support matrices for cell attachment in TISSUE ENGINEERING and GUIDED TISSUE REGENERATION uses.
Bioartificial Organs
Artificial organs that are composites of biomaterials and cells. The biomaterial can act as a membrane (container) as in BIOARTIFICIAL LIVER or a scaffold as in bioartificial skin.
Guided Tissue Regeneration
Procedures for enhancing and directing tissue repair and renewal processes, such as BONE REGENERATION; NERVE REGENERATION; etc. They involve surgically implanting growth conducive tracks or conduits (TISSUE SCAFFOLDING) at the damaged site to stimulate and control the location of cell repopulation. The tracks or conduits are made from synthetic and/or natural materials and may include support cells and induction factors for CELL GROWTH PROCESSES; or CELL MIGRATION.
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