Application of Induced Pluripotent Stem Cells in Generation of a Tissue-Engineered Tooth-Like Structure.
Summary of "Application of Induced Pluripotent Stem Cells in Generation of a Tissue-Engineered Tooth-Like Structure."
Abstract Stem cells, such as adult stem cells or embryonic stem cells, are the most important seed cells employed in tooth tissue-engineering. Even though dental-derived stem cells are a good source of seed cells for such procedures, they are not often used in clinical applications because of the limited supply. Induced pluripotent stem (iPS) cells, with their high proliferation and differentiation ability, are now considered a promising alternative. The objectives of this study were to assess the role of iPS cells in tooth tissue-engineering. We used real-time PCR to confirm that mouse iPS (miPS) cells can be induced to express both odontogenic and osteogenic gene profiles. We then established a tooth germ model and transplanted the recombinant tooth germ into mouse subrenal capsule for four weeks to reproduce early tooth organogenesis After four weeks, H&E staining results showed newly formed bone and dental pulp-like areas. Further immunohistochemical staining confirmed that osteopontin (OPN) was present in the apical part of tooth-like structure. These results demonstrate that miPS cells have the potential to differentiate into odontogenic cells, confirming that they could be a new source of seed cells for use in tooth tissue-engineering. Keywords: tissue engineering, tooth, iPS.
Affiliation
jinan, China; wenyongsdu@yahoo.com.cn.
Journal Details
This article was published in the following journal.
Name: Tissue engineering. Part A
ISSN: 1937-335X
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22676377
- DOI: http://dx.doi.org/10.1089/ten.TEA.2011.0220
Medical and Biotech [MESH] Definitions
Embryonal Carcinoma Stem Cells
The malignant stem cells of TERATOCARCINOMAS, which resemble pluripotent stem cells of the BLASTOCYST INNER CELL MASS. The EC cells can be grown in vitro, and experimentally induced to differentiate. They are used as a model system for studying early embryonic cell differentiation.
Induced Pluripotent Stem Cells
Cells from adult organisms that have been reprogrammed into a pluripotential state similar to that of EMBRYONIC STEM CELLS.
Lymphopoiesis
Formation of LYMPHOCYTES and PLASMA CELLS from the lymphoid stem cells which develop from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW. These lymphoid stem cells differentiate into T-LYMPHOCYTES; B-LYMPHOCYTES; PLASMA CELLS; or NK-cells (KILLER CELLS, NATURAL) depending on the organ or tissues (LYMPHOID TISSUE) to which they migrate.
Myelopoiesis
Formation of MYELOID CELLS from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via MYELOID STEM CELLS. Myelopoiesis generally refers to the production of leukocytes in blood, such as MONOCYTES and GRANULOCYTES. This process also produces precursor cells for MACROPHAGE and DENDRITIC CELLS found in the lymphoid tissue.
Nuclear Reprogramming
The process that reverts CELL NUCLEI of fully differentiated somatic cells to a pluripotent or totipotent state. This process can be achieved to a certain extent by NUCLEAR TRANSFER TECHNIQUES, such as fusing somatic cell nuclei with enucleated pluripotent embryonic stem cells or enucleated totipotent oocytes. GENE EXPRESSION PROFILING of the fused hybrid cells is used to determine the degree of reprogramming. Dramatic results of nuclear reprogramming include the generation of cloned mammals, such as Dolly the sheep in 1997.
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