Simultaneous quantification of metronidazole, tinidazole, ornidazole and morinidazole in human saliva.
Summary of "Simultaneous quantification of metronidazole, tinidazole, ornidazole and morinidazole in human saliva."
The aim of this study was to develop a rapid and sensitive method for the simultaneous quantification of metronidazole (MEZ), tinidazole (TNZ), ornidazole (ONZ) and morinidazole (MNZ) in human saliva. A reversed-phase high-performance liquid chromatography (HPLC) method with ultraviolet (UV) detection at 318nm was carried out on a C18 column, using a mixture of potassium dihydrogen phosphate buffer, acetonitrile, and methanol (55:15:30, v/v/v) as a mobile phase with a flow rate of 1.0ml/min. The saliva samples (100μl) were firstly deproteinized by precipitation with methanol (400μl), after which they were centrifuged and the supernatants were directly injected into the HPLC system. This method produced linear responses in the concentration ranges of 25.2-5040.0, 23.9-4790.0, 25.4-5080.0, 25.0-5000.0ng/ml with detection limits of 6.0, 17.6, 10.0 and 11.3ng/ml for MEZ, TNZ, ONZ and MNZ (S/N=3), respectively. The methods were validated in terms of intra- and inter-batch precision (within 7.3% and 9.1%, respectively), accuracy, linearity, recovery and stability. The study proved that HPLC is both sensitive and selective for the simultaneous quantification of MEZ, TNZ, ONZ and MNZ in human saliva using a single mobile phase.
Research Division of Clinical Pharmacology, The First Affiliated Hospital, Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu 210029, China.
This article was published in the following journal.
Name: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22658465
- DOI: http://dx.doi.org/10.1016/j.jchromb.2012.04.032
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