Observation of Cleavage in DNA and Nucleotides Following Oxygen K-shell Ionization by Measuring X-ray Absorption Near Edge Structure.
Summary of "Observation of Cleavage in DNA and Nucleotides Following Oxygen K-shell Ionization by Measuring X-ray Absorption Near Edge Structure."
Abstract Purpose: To investigate which type of bond is more likely to be cleaved in functional groups in DNA including the nucleobases by the K-shell ionization of oxygen of DNA, and to determine whether the production of propenal is specific to the oxygen resonant excitation. To investigate the degradation pattern, which depends on the type of nucleobase in the DNA monomer. Materials and Methods: Calf thymus DNA film and four nucleotides (dAMP, TMP, dGMP, and dCMP) films were used as samples. Soft X-rays with energy of 560 eV were used to irradiate the samples, and the changes in the X-ray absorption near edge structure (XANES) spectra during the irradiation were measured. The XANES measurements were performed by using a 0.02 eV scanning photon energy step. Results: The difference spectra for DNA and nucleotides were similar to those for pyridine deprotonation. The oxygen K-edge regions in the difference spectra were all similar apart from for the spectrum obtained at the resonant excitation energy of oxygen in DNA. The spectral change did not depend on the type of nucleotide. Conclusion: (1) Deprotonation of the nucleobase -NH is usually induced by core ionization of carbon, nitrogen, and oxygen. (2) Propenal production is specific to the oxygen K-shell resonant excitation. (3) The pattern of XANES spectral changes does not significantly depend on the type of nucleobase.
This article was published in the following journal.
Name: International journal of radiation biology
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22702534
- DOI: http://dx.doi.org/10.3109/09553002.2012.703363
Medical and Biotech [MESH] Definitions
The act of regarding attentively and studying facts and occurrences, gathering data through analyzing, measuring, and drawing conclusions, with the purpose of applying the observed information to theoretical assumptions. Observation as a scientific method in the acquisition of knowledge began in classical antiquity; in modern science and medicine its greatest application is facilitated by modern technology. Observation is one of the components of the research process.
Biological Oxygen Demand Analysis
Testing for the amount of biodegradable organic material in a water sample by measuring the quantity of oxygen consumed by biodegradation of those materials over a specific time period.
A reaction that severs one of the covalent sugar-phosphate linkages between NUCLEOTIDES that compose the sugar phosphate backbone of DNA. It is catalyzed enzymatically, chemically or by radiation. Cleavage may be exonucleolytic - removing the end nucleotide, or endonucleolytic - splitting the strand in two.
Enzymes that catalyze the cleavage of a phosphorus-oxygen bond by means other than hydrolysis or oxidation. EC 4.6.
Enzymes that catalyze the cleavage of a carbon-oxygen bond by means other than hydrolysis or oxidation. EC 4.2.
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