Downregulation of TWIK-related arachidonic acid-activated K(+) channel in the spinal cord of rats after complete bladder outlet obstruction.
Summary of "Downregulation of TWIK-related arachidonic acid-activated K(+) channel in the spinal cord of rats after complete bladder outlet obstruction."
Objectives:  To study the altered expression of TWIK-related arachidonic acid-activated K(+) channel in the L6-S1 spinal cord of rats after complete bladder outlet obstruction, and to investigate the role of TWIK-related arachidonic acid-activated K(+) channel in the neurogenic mechanism of bladder dysfunction. Methods:  Female Sprague-Dawley rats were randomly divided into a complete bladder outlet obstruction group and a sham-operated control group. Cystometry was carried out and tissues of L6-S1 spinal cord were obtained for detection of TWIK-related arachidonic acid-activated K(+) channel mRNA and protein by real-time polymerase chain reaction, western blot and immunohistochemistry. Results:  The bladder outlet obstruction rat model was established. Real-time polymerase chain reaction, western blot and immunohistochemistry showed that the expression of TWIK-related arachidonic acid-activated K(+) channel was lower in the L6-S1 spinal cord of the bladder outlet obstruction rats, compared with the control rats. Conclusions:  Downregulation of TWIK-related arachidonic acid-activated K(+) channel might enhance the excitability of the neurons and increase the sensitivity of the bladder, probably providing a new study model of overactive bladder secondary to bladder outlet obstruction.
Affiliation
Department of Urology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou Department of Urology, Shenzhen Luohu People's Hospital, Shenzhen, Guangdong, China.
Journal Details
This article was published in the following journal.
Name: International journal of urology : official journal of the Japanese Urological Association
ISSN: 1442-2042
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22709279
- DOI: http://dx.doi.org/10.1111/j.1442-2042.2012.03072.x
Medical and Biotech [MESH] Definitions
Kcnq3 Potassium Channel
A very slow opening and closing voltage-gated potassium channel that is expressed in NEURONS and is closely related to KCNQ2 POTASSIUM CHANNEL. It is commonly mutated in BENIGN FAMILIAL NEONATAL CONVULSIONS.
8,11,14-eicosatrienoic Acid
A 20-carbon-chain fatty acid, unsaturated at positions 8, 11, and 14. It differs from arachidonic acid, 5,8,11,14-eicosatetraenoic acid, only at position 5.
5,8,11,14-eicosatetraynoic Acid
A 20-carbon unsaturated fatty acid containing 4 alkyne bonds. It inhibits the enzymatic conversion of arachidonic acid to prostaglandins E(2) and F(2a).
Radiculopathy
Disease involving a spinal nerve root (see SPINAL NERVE ROOTS) which may result from compression related to INTERVERTEBRAL DISK DISPLACEMENT; SPINAL CORD INJURIES; SPINAL DISEASES; and other conditions. Clinical manifestations include radicular pain, weakness, and sensory loss referable to structures innervated by the involved nerve root.
Leukotriene B4
The major metabolite in neutrophil polymorphonuclear leukocytes. It stimulates polymorphonuclear cell function (degranulation, formation of oxygen-centered free radicals, arachidonic acid release, and metabolism). (From Dictionary of Prostaglandins and Related Compounds, 1990)
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