A proline repeat polymorphism of the Frost gene of Drosophila melanogaster showing clinal variation but not associated with cold resistance.
Summary of "A proline repeat polymorphism of the Frost gene of Drosophila melanogaster showing clinal variation but not associated with cold resistance."
Genetic polymorphisms underlying adaptive shifts in thermal responses are poorly known even though studies are providing a detailed understanding of these responses at the cellular and physiological levels. The Frost gene of Drosophila melanogaster is a prime candidate for thermal adaptation; it is up-regulated under cold stress and knockdown of this gene influences cold resistance. Here we describe an amino-acid INDEL polymorphism in proline repeat number in the structural component of this gene. The two main repeats, accounting for more than 90% of alleles in eastern Australia, show a strong clinal pattern; the 6P allele was at a high frequency in tropical locations, and the 10P allele was common in temperate populations. However, the frequency of these alleles was not associated with three different assays of cold resistance. Adult transcription level of Frost was also unrelated to cold resistance as measured through post chill coma mobility. The functional significance of the proline repeat polymorphism therefore remains unclear despite its clinal pattern. The data also demonstrate the feasibility of using Roche/454 sequencing for establishing clinal patterns.
Affiliation
Department of Genetics, Bio21 Institute, The University of Melbourne, Melbourne, Victoria, Australia School of Biological Sciences, Monash University, Melbourne, Victoria, Australia.
Journal Details
This article was published in the following journal.
Name: Insect molecular biology
ISSN: 1365-2583
Pages:
Links
- PubMed Source: http://www.ncbi.nlm.nih.gov/pubmed/22708613
- DOI: http://dx.doi.org/10.1111/j.1365-2583.2012.01149.x
Medical and Biotech [MESH] Definitions
Drosophila Proteins
Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.
Entomobirnavirus
A genus of RNA viruses of the family BIRNAVIRIDAE infecting fruitflies. Transmission is horizontal and there are no known vectors. Drosophila melanogaster is the natural host and the type species is Drosophila X virus.
Drosophila Melanogaster
A species of fruit fly much used in genetics because of the large size of its chromosomes.
Proline Oxidase
The first enzyme of the proline degradative pathway. It catalyzes the oxidation of proline to pyrroline-5-carboxylic acid in the presence of oxygen and water. The action is not reversible. The specific activity of proline oxidase increases with age. EC 1.5.3.-.
Son Of Sevenless Proteins
A class of RAS GUANINE NUCLEOTIDE EXCHANGE FACTORS that are genetically related to the Son of Sevenless gene from DROSOPHILA. Sevenless refers to genetic mutations in DROSOPHILA that cause loss of the R7 photoreceptor which is required to see UV light.
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